计算分析以了解来自Bacillus spp的纤维解酶的结构-功能特性,以便将其有效地集成到工业应用中
Nitisha Boro1, Pedro Alexandrino Fernandes2, Ashis K Mukherjee1,3
1Microbial Biotechnology and Protein Research Laboratory, Department of Molecular Biology and Biotechnology, Tezpur University, Tezpur, 784028, Assam, India.
Heliyon
|July 26, 2024
概括
来自Bacillus sp. 的纤维分解酶. 显示出预防血栓形成的潜力. 计算分析确定了稳定的酶序列 (JF739176.1,MF677779.1),具有用于药物开发的治疗性质.
科学领域:
- 生物化学 生物化学
- 计算生物学 计算生物学
- 酶学 是一种酶学.
背景情况:
- 来自*Bacillus*物种的纤维解酶正在研究它们在预防血栓形成方面的治疗潜力.
- 对酶性质的计算分析可以提高它们的治疗效果.
研究的目的:
- 通过计算分析来自 *Bacillus* sp. 的纤维解酶. 为了改进治疗应用.
- 识别具有增强的催化活性和稳定性的特定酶序列.
主要方法:
- 从NCBI数据库中检索并对齐纤维解酶的核酸和蛋白质序列.
- 使用各种计算工具 (ExPASy-ProtParam,MEME,SOPMA等) 进行计算. 用于生理化学,结构和功能分析.
- 通过与已发表的实验结果进行比较,验证了*in-silico*数据.
主要成果:
- 在60个蛋白酶酶中确定了49个具有保存的催化三合体 (Asp196,His242,Ser569).
- 酶表现出高的热稳定性,预测的不稳定性指数在1.94-37.77之间,酸性指数在68.9-93.41之间.
- 鉴定了特定的酶序列 (JF739176.1,MF677779.1),由于稳定性和活性,它们是突变酶发展的有希望的候选者.
结论:
- 计算分析为治疗开发提供了对纤维解酶特性有价值的见解.
- 酶序列JF739176.1和MF677779.1显示出适用于制造针对血栓形成的改善治疗剂的特性.
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