相关实验视频

Updated: Jun 19, 2025

Use of a Hanging-weight System for Liver Ischemia in Mice

Use of a Hanging-weight System for Liver Ischemia in Mice

Published on: August 7, 2012

皮姆-1 激酶通过调节与动态相关的蛋白1 1 的动态来保护肝脏免受缺血再流损伤

Yan-Dong Sun¹, Qing-Guo Xu¹, De-Shu Dai¹

¹Organ Transplantation Center, The Institute of Transplantation Science, The Affiliated Hospital of Qingdao University, Qingdao, Shandong Province, China.

|July 26, 2024

概括

原始瘤基因血清蛋白/氨酸蛋白激酶 (Pim-1) 保护在缺血-再输液 (IR) 后的肝损伤. 增加的Pim-1稳定了线粒体并减少了细胞死亡,为肝脏IR损伤提供了一个新的治疗点.

科学领域:

肝病学肝病学是一种肝病学.
分子生物学分子生物学
细胞生物学细胞生物学

背景情况:

肝缺血-再输血 (IR) 损伤是肝移植的一个主要挑战.
目前肝脏IR损伤的治疗方法不足,需要新的治疗策略.

研究的目的:

为了研究原瘤基因血清蛋白/氨酸蛋白激酶 (Pim-1) 在肝脏IR损伤中的作用.
阐明Pim-1在肝脏中的功能背后的分子机制.

主要方法:

已确立的小鼠肝脏IR *in vivo* 和MIHA细胞低氧复氧化 *in vitro* 模型.
评估了Pim-1表达水平,并将它们与肝损伤标志物 (ALT,AST) 相关联.
研究了Pim-1对线粒体动态 (Drp1酸化,裂变) 和亡的影响.
研究了Pim-1的表观遗传调节,特别是基因组H3氨酸9三甲基化.

主要成果:

在肝脏IR之后,Pim-1表达得到了上调.
增加的Pim-1表达与血清ALT和AST水平相反相关,表明具有保护作用.
皮姆-1调节了Drp1酸化,导致线粒体膜稳定,减少裂变和减少亡.
在肝脏IR期间,发现Pim-1表达的升高依赖于组素H3氨酸9三甲基化.

结论:

皮姆-1在减轻肝脏IR损伤方面发挥着关键的保护作用.
皮姆-1通过调节线粒体完整性和减少亡来发挥其保护作用.
表观遗传修饰,特别是基因组H3 lysine 9三甲基化,参与调节Pim-1的表达,以响应IR.
向Pim-1代表了一种有前途的新疗法策略,用于管理肝脏IR损伤.

关键词:

细胞生理学细胞生理学内分泌学在内分泌学.

更多相关视频

Improved Rodent Model of Myocardial Ischemia and Reperfusion Injury

Improved Rodent Model of Myocardial Ischemia and Reperfusion Injury

Published on: March 7, 2022

Mechanism of Kemeng Fang's Inhibition of Podocyte Apoptosis in Rats with Membranous Nephropathy through the PI3K/AKT Signaling Pathway

Mechanism of Kemeng Fang's Inhibition of Podocyte Apoptosis in Rats with Membranous Nephropathy through the PI3K/AKT Signaling Pathway

Published on: August 23, 2024

相关概念视频

Regulation of the Unfolded Protein Response

Regulation of the Unfolded Protein Response

Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...

PI3K/mTOR/AKT Signaling Pathway

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a...

The JAK-STAT Signaling Pathway

The JAK-STAT Signaling Pathway

Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as SH2...

Phosphoinositides and PIPs

Phosphoinositides and PIPs

Phosphoinositides are a group of phospholipids containing a glycerol backbone with two fatty acid chains and a phosphate attached to a myoinositol sugar ring. The inositol head group extends into the cytoplasm, where it is modified by adding phosphate groups to form phosphatidylinositol phosphates or PIPs.
Different phosphoinositides are synthesized and recruited on the cytosolic face of the plasma membrane. The localization of specific phosphoinositides concentrated in separate membrane...

Interactions Between Signaling Pathways

Interactions Between Signaling Pathways

Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...

Abnormal Proliferation

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...