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Updated: Jun 19, 2025

Protocol for Recombinant RBD-based SARS Vaccines: Protein Preparation, Animal Vaccination and Neutralization Detection

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Published on: May 2, 2011

使用尖端蛋白受体结合域作为诱导SARS-CoV-2中和的抗原的多重复合蛋白的开发和表征

Veronica A de Lima¹, João P S Nunes¹, Daniela S Rosa¹

¹Department of Microbiology, Immunology and Parasitology, Universidade Federal de São Paulo, São Paulo, São Paulo, Brazil.

Immunity, inflammation and disease

|July 26, 2024

概括

为SARS-CoV-2开发了一种新型重组受体结合域 (RBD) 蛋白质,该蛋白质展示了三元和六元结构. 这种蛋白质有效地引起中和抗体和细胞免疫反应,提供了对抗COVID-19的新工具.

科学领域:

病毒学病毒学
免疫学免疫学免疫学
生物技术是生物技术.

背景情况:

严重急性呼吸系统综合征冠状病毒2 (SARS-CoV-2) 是导致COVID-19的原因.
病毒的表面蛋白 (S蛋白) 对于通过 ангиотензин转化酶2 (ACE2) 结合进入细胞至关重要.
S蛋白的受体结合域 (RBD) 是疫苗和诊断的关键目标.

研究的目的:

开发一种稳定,构成性表达的复合RBD蛋白,用于SARS-CoV-2.
利用这种蛋白质作为一种替代性免疫原和COVID-19的诊断工具.

主要方法:

具有三元化域和净化标签的工程RBD蛋白.
在HEK293细胞中稳定表达和通过染色学净化.
结构性特征,小鼠免疫和免疫反应的评估 (抗体标位,中和,IFN-γ产生).

主要成果:

纯化的重组RBD蛋白形成了trimers和hexamers,结合ACE2.
用RBD蛋白和AS03辅助剂进行免疫接种诱导了高抗体标位 (高达1:50,000).
塞拉证明了ACE2结合抑制和中和SARS-CoV-2 (高达1:2000),诱导T细胞反应.

结论:

一种新型的多重体RBD蛋白诱导了对SARS-CoV-2的保护性幽默和细胞免疫力.
这种工程RBD蛋白质是对抗COVID-19的武器库的宝贵补充.
潜在的应用包括替代免疫原和诊断抗原.

关键词:

在COVID - 19中.在SARS-CoV-2中.中和抗体的抗体中和.蛋白质 S 蛋白质 S 是一种蛋白质.受体结合域是一个受体结合域.

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Conjugated Proteins

Conjugated Proteins

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Nucleoproteins are protein complexes that contain nucleic acids, categorized as deoxyribonucleoproteins (DNPs) or ribonucleoproteins (RNPs) respectively. The nucleosome is a typical example of a DNP where nuclear DNA is associated with histone proteins. The major antigen for the Covid-19 virus SARS-CoV is an RNP that is critical...