相关实验视频

Updated: Jun 19, 2025

Author Spotlight: Investigating the Pathophysiology of Eosinophilic Esophagitis

Author Spotlight: Investigating the Pathophysiology of Eosinophilic Esophagitis

Published on: May 10, 2024

纤维细胞亚种群对异性贡献细胞间通信在酸性食道炎中

Tao Li¹, Matthew Salomon², Ling Shao³

¹Swallowing and Esophageal Disorders Center, Division of Gastrointestinal and Liver Diseases, Department of Medicine, Keck School of Medicine of USC, Los Angeles, CA 90089, USA.

|July 26, 2024

概括

这项研究确定了异性食道炎 (EoE) 中不同的纤维细胞群,透露了它们通过细胞间通信,特别是通过细胞外矩阵信号传递在疾病进展和纤维化中的关键作用.

科学领域:

免疫学免疫学免疫学
胃肠病学胃肠病学
细胞生物学细胞生物学

背景情况:

性食道炎 (EoE) 是一种过敏性炎症疾病,其特征是食道纤维化.
纤维细胞异质性及其在EoE病原体中的特定作用尚未得到充分理解.

研究的目的:

用单细胞RNA测序来识别和描述EoE中不同的纤维细胞亚群.
研究这些纤维细胞群体的转录组形状,疾病特异性途径和细胞与细胞相互作用.
使用IL-13处理的纤维细胞培养物建模活性EoE.

主要方法:

从EoE食道活检中分析公开可用的单细胞RNA测序数据 (GSE201153).
基于基因表达的纤维细胞亚群 (F_A和F_B) 的鉴定,包括ACTA2.
使用IL-13处理的纤维细胞培养物进行活性EoE的体外建模.

主要成果:

至少确定了两个不同的纤维细胞群 (F_A和F_B),其中F_B更为普遍.
在F_B中,表皮层-介质细胞转换 (EMT) 在活跃和缓解的EoE中都被上调.
在ACTA2-纤维细胞中,IL-13治疗上调了与细胞外基因组 (ECM) 相关的基因.
纤维细胞群F_A和F_B是细胞-细胞通信网络的关键贡献者,在活跃的EoE中,ECM-受体相互作用占主导地位.

结论:

这项研究在EoE中确定了至少两个纤维细胞亚群,部分通过ACTA2表达来区分.
纤维细胞,特别是F_B子组,通过细胞间相互作用,主要通过ECM受体信号传递,在EoE病原发生中发挥关键作用.

关键词:

细胞细胞通信细胞细胞通信埃索诺菲尔性食道炎 (eosinophilic esophagitis) 是一种导致食道炎的疾病.纤维细胞亚种群.纤维细胞的细胞.路径分析路径分析分析单细胞RNA测序数据分析.

更多相关视频

Functional Characterization and Visualization of Esophageal Fibroblasts Using Organoid Co-Cultures

Functional Characterization and Visualization of Esophageal Fibroblasts Using Organoid Co-Cultures

Published on: January 6, 2023

Isolation of Myofibroblasts from Mouse and Human Esophagus

Isolation of Myofibroblasts from Mouse and Human Esophagus

Published on: January 18, 2015

相关概念视频

Introduction to Fibroblasts

Introduction to Fibroblasts

Rudolph Virchow discovered spindle-shaped cells called fibroblasts in 1858. Inactive fibroblasts, called fibrocytes, become activated by various stimuli, such as growth factors and inflammatory cytokines. Activated fibroblasts play a crucial role in wound healing, inflammation, formation of new blood vessels, and cancer progression. Uncontrolled activation of fibroblasts results in fibrosis, the excess deposition of fibrous tissue, which can lead to scarring and affect normal organs. This...

Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal

Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal

Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.