相关实验视频

Updated: Jun 19, 2025

Effect of Hyaluronic Acid 35 kDa on an In Vitro Model of Preterm Small Intestinal Injury and Healing Using Enteroid-Derived Monolayers

Effect of Hyaluronic Acid 35 kDa on an In Vitro Model of Preterm Small Intestinal Injury and Healing Using Enteroid-Derived Monolayers

Published on: July 28, 2022

内源性 Hyaluronan 促进肠道平衡,并防止小鼠死性肠球炎

Jeffery V Eckert¹, Karni S Moshal¹, Kathryn Burge¹

¹Department of Pediatrics, Division of Neonatal-Perinatal Medicine, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA.

|July 26, 2024

概括

氨酸 (HA) 对于肠道发育和预防死性肠球炎 (NEC) 的保护至关重要. 在小鼠中抑制HA信号,损害了肠道发育,增加了类似NEC的损伤,突出显示了HA的保护作用.

科学领域:

胃肠病学胃肠病学
发展生物学发展生物学
免疫学免疫学免疫学

背景情况:

结核性肠球炎 (NEC) 是早产婴儿的严重胃肠道疾病.
它的发展涉及肠道屏障功能障碍,微生物失衡和免疫失调.
内源性氨酸 (HA) 在肠道发育和NEC病变发生中的作用尚不清楚.

研究的目的:

研究内源性氨酸 (HA) 在新生小鼠肠道发育中的功能.
评估抑制HA信号对NEC类肠道损伤的影响.

主要方法:

新生小鼠接受了PEP1的治疗,这是一种抑制HA受体相互作用的,或是混杂的控制.
通过测量小长度,密室深度,细胞增殖和细胞分化来评估肠道发育.
在接受治疗和对照组中评估了对NEC类伤害和死亡率的敏感性.

主要成果:

PEP1治疗显著抑制了肠道发育,减少了小长度,密室深度和上皮细胞增殖.
在接受PEP1治疗的小鼠中,密室裂变活性下降.
用PEP1治疗的小鼠对NEC类损伤的敏感性增加,死亡率更高,组织学损伤更严重.

结论:

内源性氨酸在支持正常肠道发育方面发挥着至关重要的作用.
抑制HA信号破坏了肠道的完整性,并增加了对NEC类损伤的敏感性.
针对HA相互作用可能为预防或治疗NEC提供治疗策略.

关键词:

在NEC模型中,NEC模型是:密码裂变裂变是什么意思氨酸 (HA) 是一种氨酸.肠道的发育肠道的发育死性肠球炎 (NEC) 是一种过早出生的婴儿是早产的

更多相关视频

Systematic Scoring Analysis for Intestinal Inflammation in a Murine Dextran Sodium Sulfate-Induced Colitis Model

Systematic Scoring Analysis for Intestinal Inflammation in a Murine Dextran Sodium Sulfate-Induced Colitis Model

Published on: February 14, 2021

In Vitro Apical-Out Enteroid Model of Necrotizing Enterocolitis

In Vitro Apical-Out Enteroid Model of Necrotizing Enterocolitis

Published on: June 8, 2022

相关概念视频

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...

Renewal of Intestinal Stem Cells

Renewal of Intestinal Stem Cells

The intestinal epithelial lining rapidly renews every 4 to 5 days. The renewal is facilitated by intestinal stem cells (ISCs) located at the base of the crypt– a gland located at the bottom of each villus. ISCs divide asymmetrically to form new stem cells and progenitor daughter cells. The daughter cells are called transit-amplifying (TA) cells which move upwards along the crypt and either differentiate into absorptive cells– the enterocytes or secretory cells– including the...