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一个编码3 - 基-3 - 甲基氨酸-CoA降低酶 (HMGR) 的基因分子特征和表达分析,来自双极星,一种产生的Ophiobolin A的真菌

Jianping Zhang¹, Ke Yang², Wei Tang¹

¹State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China.

Journal of fungi (Basel, Switzerland)

|July 26, 2024

概括

奥菲博林A是一种真菌的塞斯特烯,具有多种生物活性. 研究人员鉴定和表征了来自Bipolaris eleusines的3-hydroxy-3-methylglutaryl-CoA减少酶 (HMGR) 基因,揭示了其由甲基斯莫纳酸诱导的表达.

科学领域:

菌类学菌类学是指菌类学.
生物化学生物化学
分子生物学分子生物学

背景情况:

奥菲博林A是一种真菌塞斯特烯,具有显著的除草剂,杀菌剂,杀菌剂和细胞毒性.
了解ophiobolin A的生物合成对于探索其生物活动和潜在应用至关重要.

研究的目的:

为了分离和描述编码3 - 基-3 - 甲基酸-CoA减少酶 (HMGR) 的基因,该基因参与Bipolaris eleusines中的ophiobolin A生物合成.
在甲基斯蒙酸诱导下研究BeHMGR基因的表达模式.

主要方法:

转录组测序被用来识别与塞斯特烯生物合成相关的基因.
快速放大cDNA末端 (RACE) 技术被用于隔离全长的BeHMGR基因.
使用定量光PCR分析基因表达水平.

主要成果:

B. eleusines HMGR基因 (BeHMGR) 的全长cDNA为3906 bp,编码了1157个氨基酸.
序列分析显示,BeHMGR与已知的HMGR具有很高的同质性,具有保存的HMG-CoA结合和NADP (H) 结合基因.
BeHMGR基因表达被甲基斯酸盐显著诱导,在6小时达到峰值.

结论:

已识别的BeHMGR基因在ophiobolin A生物合成的美酸途径中发挥着关键作用.
甲基斯蒙酸是B. eleusines. 中BeHMGR基因表达的有效诱导剂.

关键词:

在 RACE 技术方面,克隆,就是克隆人类.甲基斯蒙酸盐 (MJ) 是一种植物毒素是一种植物毒素.转录基因组测序的转录基因组测序

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