使用MALDI-TOF识别食物腐烂真菌MS:光谱数据库开发和应用到复杂物种
Nolwenn Rolland1,2, Victoria Girard1, Valérie Monnin1
1bioMérieux, R&D Microbiologie, Route de Port Michaud, F-38390 La Balme les Grottes, France.
Journal of fungi (Basel, Switzerland)
|July 26, 2024
概括
矩阵辅助激光脱/电离飞行时间质谱 (MALDI-TOF MS) 增强了食品安全中的真菌鉴定. 扩展VITEK® MS数据库提高了准确性,特别是在区分密切相关的物种.
科学领域:
- 食品微生物学 食品微生物学
- 分析化学是一种分析化学.
- 菌类学 菌类学是指菌类学.
背景情况:
- 会导致大量的食物损失,并产生真菌毒素.
- 准确的真菌鉴定对于食品安全和质量至关重要.
- MALDI-TOF MS提供快速可靠的真菌鉴定.
研究的目的:
- 将VITEK® MS数据库扩展到与食品相关的真菌物种.
- 评估扩展的真菌识别数据库的性能.
- 评估MALDI-TOF MS在复杂物体内区分物种的能力.
主要方法:
- 在VITEK® MS数据库中添加了380种酵母和菌菌株 (51个属,133个物种).
- 包括来自五个主要真菌复合体的物种 (例如,阿斯伯吉卢斯,Fusarium,Colletotrichum).
- 通过交叉验证和对78个隔离物进行外部测试,验证了数据库性能.
主要成果:
- 通过交叉验证实现了96.55%的正确识别.
- 在外部验证中达到90.48%的正确识别.
- 证明了MALDI-TOF MS在区分密切相关的真菌物种方面的能力.
结论:
- 扩展的VITEK® MS数据库提高了真菌识别的准确性.
- MALDI-TOF MS在工业环境中有效用于常规的真菌识别.
- 这种技术有助于确保食品安全和质量,通过精确检测真菌.
关键词:
亚斯珀吉洛斯 (Aspergillus) 是一种植物.科莱托特里克 (Collettotrichum) 是一种有机物.这里是 Fusariumarium .粘膜 粘膜是一种粘膜.隐秘的物种是神秘的物种.有丝状真菌的真菌.酵母酵母是一种酵母.更多相关视频
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