tps1删除和过度表达对Trichoderma atroviride中的代谢物的影响
Xinyue Wang1, Wenzhe Li1, Shuning Cui1
1Ecology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250013, China.
Journal of fungi (Basel, Switzerland)
|July 26, 2024
概括
这项研究探讨了tps1基因在Trichoderma atroviride中的作用,揭示了它在类合成中的重要性. 操纵tps1基因表达改变了烯的产生,但对真菌活动和植物生长的影响很小.
科学领域:
- 菌类学 菌类学是指菌类学.
- 生物化学 生物化学
- 自然产品 化学 化学
背景情况:
- 烯酸是重要的自然产品,具有多种工业应用.
- 菌的类蛋白产量很大,但菌的类合成酶还没有得到充分的研究.
- tps1基因是类生物合成途径的关键组成部分.
研究的目的:
- 为了研究tps1基因在Trichoderma的功能,ATROVIRIDE HB20111.11.
- 分析tps1基因缺失和过度表达对烯代谢物概况的影响.
- 为了评估TPS1蛋白作为基烯循环酶的作用.
主要方法:
- 基因组挖掘以隔离tps1基因.
- 构建tps1-基因删除 (Δtps1) 和过度表达 (Otps1) 菌株.
- 转录水平分析和HS-SPME-GC-MS用于代谢物概况.
主要成果:
- 在野生型,Δtps1和Otps1菌株之间观察到类代谢物的显著变化.
- Δtps1菌株显示减少了甲挥发性有机化合物 (VOC),而Otps1菌株过度生产了特定的甲.
- 对TPS1蛋白质的分析表明了四烯循环酶的活性.
结论:
- 在T. atroviride中,tps1基因对烯合成至关重要,但不是唯一的决定因素.
- 与类化合物相关的活动,如真菌抑制和植物生长促进,很可能是多因素的.
- 这项研究为了解真菌类代谢途径提供了基础.
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