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Updated: Jun 19, 2025

Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems
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Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems

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来自LC-MS的可交换的数量 来自重水代谢标记样本的重水数据

Henock M Deberneh1, Michael E Taylor2, Agnieszka K Borowik2

  • 1Department of Biochemistry and Molecular Biology The University of Texas Medical Branch 301 University of Blvd, Galveston, Texas 77555, United States.

Journal of the American Society for Mass Spectrometry
|July 26, 2024
PubMed
概括

这项研究引入了新方法来准确计算合物 (D2O) 用于测量蛋白质合成速率. 最好的方法提高了在特定的实验条件下确定每个氨基酸的标签位置的准确性.

关键词:
重水代谢标签重水代谢标签乳标签站点的数量.蛋白质的半衰期 蛋白质的半衰期蛋白质营业额的变化标签丰富的量化方法时间课程建模模型

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相关概念视频

¹H NMR of Labile Protons: Deuterium (²H) Substitution00:48

¹H NMR of Labile Protons: Deuterium (²H) Substitution

This lesson illustrates the role of deuterium substitution in simplifying the NMR spectrum of compounds comprising labile protons. One method employed is the use of deuterium. Amongst the three isotopes of hydrogen, deuterium (2H) has a nucleus composed of one proton and one neutron. When the D2O solvent is added to a pure dry ethanol solution, its labile proton is substituted with deuterium.
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