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miR-21/SMAD2 在产卵的卵泡粒状细胞中因脂聚糖暴露而导致的孕激素合成降低中参与

Xinyi Guo^1,2,3, Shijia Ying^1,3, Huiping Xiao^1,2

¹MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.

|July 26, 2024

概括

脂多糖 (LPS) 通过影响性类固醇激素的产生,损害的卵巢功能. 微RNA-21 (miR-21) 起着关键作用,降低SMAD2的下调和降低孕激素的合成.

科学领域:

生殖生物学生殖生物学
分子内分泌学分子内分泌学
动物科学动物科学

背景情况:

来自格拉姆阴性细菌的脂聚糖 (LPS) 会导致生殖系统损伤,影响的生育能力.
卵巢功能障碍是子生殖能力下降的重要原因.
由LPS引起的性类固醇激素合成损害的精确分子机制尚不清楚.

研究的目的:

为了研究微RNAs (miRNAs) 在繁殖暴露于LPS在峰值产卵期间的作用.
阐明LPS诱导的卵巢功能障碍在的基础上的分子机制.

主要方法:

在产卵高峰期的20只扬州被注射了LPS (0,24,36小时).
状颗粒细胞被收集用于RNA测序,以分析差异表达的miRNAs.
生物信息学分析发现miR-21和SMAD2是关键参与者.

主要成果:

LPS改变了层次的毛囊的外观.
发现miR-21可以降低SMAD2表达和LPS处理的粒细胞 (GCs) 中的孕激素 (P4) 生产.
过度表达miR-21或SMAD2沉默通过降低STAR和CYP11A1表达来抑制性类固醇生物合成,而miR-21下调则加剧了LPS诱导的P4下降.

结论:

miR-21通过SMAD2下调参与LPS诱导的雌激素合成在粒细胞中的调节.
这项研究为预防LPS诱导的子功能障碍提供了理论基础.

关键词:

子子子子颗粒状细胞是颗粒状细胞.脂多糖类糖化物脂多糖类糖化物据报道,米R-21的发射器为米R-21 孕激素是孕激素的一种.

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