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再组合的Ixodes scapularis卡尔雷蒂库林结合了补充蛋白,但没有保护Borrelia burgdorferi免受补充杀伤

Moiz Ashraf Ansari¹, Thu-Thuy Nguyen¹, Klaudia Izabela Kocurek²

¹Department of Veterinary Pathobiology, School of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX 77843, USA.

Pathogens (Basel, Switzerland)

|July 26, 2024

概括

感染的唾液中的 Ixodes scapularis calreticulin (CRT) 可能有助于 Borrelia burgdorferi 逃避免疫反应. 这种蛋白质促进细菌的复制,可能有助于莱姆病的传播和殖民.

科学领域:

免疫学免疫学免疫学
寄生虫学的寄生虫学
微生物学微生物学

背景情况:

头皮 (Ixodes scapularis) 通过Borrelia burgdorferi传播莱姆病 (LD).
的唾液蛋白质,如卡尔雷蒂库林 (CRT),在传播中发挥作用.
在受感染的I. scapularis灵中发现CRT水平升高.

研究的目的:

研究I. scapularisCRT和人类血蛋白之间的相互作用.
探索CRT在保护B. burgdorferi免受补充介导杀戮中的作用.
确定CRT对B. burgdorferi复制的作用.

主要方法:

蛋白质的差分沉 (DiffPOP) 和LC-MS/MS分析.
评估了重组I. scapularisCRT (rIxsCRT) 对补充组件 (C1,C3,C5,C9) 的结合.
通过经典和莱克通路测量了膜攻击复合体 (MAC) 沉积.
评估了rIxsCRT对B. burgdorferi在血清和复制中的存活率的影响.

主要成果:

rIxsCRT结合了C1复合体,但没有抑制经典补充途径MAC沉积.
rIxsCRT结合C3,C5和C9,通过莱克通路减少MAC沉积.
rIxsCRT不能保护B. burgdorferi免受血清诱导的杀死,但可以促进其复制.

结论:

I. scapularis的CRT可能会促进B. burgdorferi的传播和殖民.
CRT可能作为诱来激活补充剂并增强咬伤部位的细菌复制.
CRT是了解和预防莱姆病的潜在目标.

关键词:

波雷莉亚的形虫 (Borrelia burgdorferi) 是一种形虫.它们是 Ixodes scapularis 头皮.补充级联级联的补充.卡尔雷蒂库林 (calreticulin) 是一种

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