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活跃肺结核与潜伏肺结核:哪一个是合适的区分生物标志物?
Rizk Sayad R Sarhan1, Omnia Y Habashy2, Raafat R Mohammed3
1Department of Internal Medicine, Faculty of Medicine, Benha University. rizk.r.sarhan72023@gmail.com.
血微RNA (miR-) 水平可以帮助患者区分潜伏肺结核 (LTB) 和活跃结核 (ATB). 特定的miRs,如miR-378-5p和miR-4523-5p,显示出作为生物标志物用于识别LTB.
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 免疫学 免疫学 免疫学
背景情况:
- 结核病 (TB) 诊断依赖于临床,放射学和微生物学方法.
- 区分潜伏结核病感染 (LTB) 和活跃结核病 (ATB) 对于有效的治疗和传播控制至关重要.
- 微RNAs (miRs) 是小型非编码RNAs,它们调节基因表达,并与包括结核病在内的各种疾病有关.
研究的目的:
- 研究血微RNA表达水平作为生物标志物的潜力,以区分健康对照 (HC),LTB患者和ATB患者.
- 识别特定的microRNAs,可以作为肺结核的诊断或预后指标.
主要方法:
- 从三个组收集血样本:30名ATB患者,30名LTB患者 (家庭接触者QFT-GIT阳性) 和30名HC.
- 使用逆转录定量聚合酶链反应 (RT-qPCR) 来量化微RNA表达水平.
- 使用统计分析,包括接收器操作特征 (ROC) 曲线分析和多变量回归来评估诊断能力.
主要成果:
- 与LTB和HC样本相比,ATB样本中的血miR-150-5p显著下调.
- 与HC和LTB样本相比,ATB样本中的miR-155-5p和miR-378-5p显著过度表达.
- 与ATB和HC样本相比,miR-4523-5p在LTB样本中显著上调,这表明它在识别LTB中的潜在作用.
结论:
- 肺结核感染导致血微RNA表达的放松调节,与感染严重程度相关.
- 血中的miR-378-5p和miR-4523-5p水平可以作为可靠的组合生物标志物来识别LTB患者.
- 鉴定的microRNAs显示出作为区分结核病感染状态的非侵入性生物标志物的潜力.
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