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肠上皮质分化和屏障功能通过AMPK通路激活通过Cynara cardunculus L.叶片提取物在体外促进

Claudia Muscarà¹, Antonio Speciale¹, Maria Sofia Molonia¹

¹Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Messina, Italy.

Natural product research

|July 26, 2024

概括

来自Cynara cardunculus L.叶的多 (CCLE) 通过改善细胞分化和紧密结合组装来增强肠道屏障功能. 这表明CCLE是肠道健康的有希望的自然方法.

科学领域:

胃肠病学胃肠病学
细胞生物学细胞生物学
药理学药理学是指药理学的学科.

背景情况:

肠上皮质屏障功能障碍,或漏肠,允许有害物质透,触发免疫反应.
聚因其增强肠道屏障完整性和防止漏肠的潜力而得到认可.
Cynara cardunculus L. 叶子是生物活性多的丰富来源.

研究的目的:

研究Cynara cardunculus L.叶 (CCLE) 富含多的提取物对肠道屏障功能的保护作用背后的体外分子机制.
评估CCLE对人类肠道上皮细胞系Caco-2的完整性的影响.
阐明参与CCLE对肠道屏障的有益影响的信号通路.

主要方法:

利用Caco-2人类上皮细胞来建模肠道屏障.
通过测量跨表皮电阻 (TEER) 和对光素的细胞透性来评估肠道屏障功能.
使用Ca2+开关试验评估紧结 (TJ) 组件,并分析了TJ蛋白表达.
通过性酸酶活性测量了上皮细胞分化.
研究了AMP激活蛋白激酶 (AMPK) 途径的作用.

主要成果:

CCLE治疗显著增加了TEER,并降低了分化的Caco-2细胞中的细胞透性.
CCLE促进了紧密结合组件的更快恢复,并刺激了上皮细胞分化.
CCLE上调性酸酶活性和紧结蛋白.
观察到的CCLE的保护作用与AMPK通路的激活有关.

结论:

来自Cynara cardunculus L.叶 (CCLE) 的富含多的提取物有效地增强了肠道屏障功能和完整性.
CCLE促进了上皮细胞分化,并改善了紧密结合组合,可能是通过AMPK通路激活.
农业副产品Cynara cardunculus L. 叶片是多的宝贵来源,可以支持肠道健康.

关键词:

在 AMPK 路径上.卡科-2 细胞辛纳拉卡登克卢斯 L 卡登克卢斯 L 漏肠综合征是什么意思漏肠综合征自然产品是自然产品的产品.蛋白质表达蛋白质表达紧密的交叉路口紧密的连接

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