牛肉中的肝转录分析:食内菌感染高菌种子的影响
Gastón F Alfaro1, Valentino Palombo2, MariaSilvia D'Andrea2
1Department of Animal Sciences, Auburn University, Auburn, AL, United States of America.
PloS one
|July 26, 2024
概括
在肉牛中,食用感染了内菌的高的种子会激活肝脏代谢途径. 这表明,牛激活了应对高毒素的机制,影响了肝功能和能量消耗.
科学领域:
- 动物科学动物科学
- 生物化学 生物化学
- 毒理学 毒理学 毒理学
背景情况:
- 由内菌感染的种子引起的高身毒性,对牲畜产生负面影响.
- 了解毒性病的分子机制对于牛的健康至关重要.
研究的目的:
- 为了研究内菌感染 (E+) 高菌种子摄入对生长中的肉牛肝转录组的影响.
- 通过使用RNA测序来识别受E+高消耗影响的代谢途径.
主要方法:
- 在30天的时间里,成长中的Angus × Simmental公牛和公牛被养了无内菌体 (E-) 或E+高的虫种子.
- 在第30天通过活检收集肝脏组织进行RNA测序 (RNA-seq) 分析.
- 饮食摄入量包括百慕大草干草,一种营养补充剂,以及E+或E-高种子,每天20μg ergovaline/kg体重.
主要成果:
- 摄入E+高的摄入对肝组织中关键的代谢途径进行了上调.
- 激活的途径包括氧化酸化,核糖体生物发生,内分泌网膜蛋白质加工和亡.
- "热生成"路径的升级表明肝脏的能量消耗增加.
结论:
- 消费E+高的便会触发肉牛的适应性肝脏反应.
- 这些反应旨在抵消肝功能受损并管理增加的能量需求.
- 这些发现有助于我们更好地了解在高毒性期间牛肉牛肝脏代谢的情况.
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