相关实验视频
Updated: Jun 19, 2025

11:51
Facile Preparation of 4-Substituted Quinazoline Derivatives
Published on: February 15, 2016
11.9K
含有 (3R,4S) 立体配置的昆类类化合物的以埃斯特结合物的生物合成
Yujing Zeng1, Tiantian Lu1, Shuya Ren1
1School of Marine Sciences, Sun Yat-sen University, Guangzhou 510006, China.
Organic letters
|July 26, 2024
概括
这项研究揭示了Aspergillus oryzae如何产生阿斯佩拉林,强大的鱼类病原体抑制剂. 确定了像AplB和AplJ这样的关键酶,使新的维里迪卡衍生物的潜在生物催化成为可能.
科学领域:
- 自然产品生物合成 自然产品生物合成
- 微生物生物化学 微生物生物化学
- 酶工程是什么? 酶工程是什么?
背景情况:
- 阿斯佩拉林是一种新型的维里迪卡天然产品类.
- 这些化合物对鱼类病原体具有强大的抑制活性.
研究的目的:
- 为了阐明阿斯佩拉林在异质宿主阿斯佩拉林或yzae NSAR1.1.中的生物合成.
- 为了确定参与阿斯佩拉林形成的关键酶.
- 探索已识别的酶对生物催化物的潜力.
主要方法:
- 在Aspergillus oryzae中异质表达NSAR1.
- 对FAD依赖的单氧基酶AplB.B.的酶性表征.
- 对NRPS AplJ活动的分析.
- 对其他修饰酶 (AplF,AplN,AplE) 的生物化学测定.
主要成果:
- 已识别的FAD-依赖单氧基酶AplB在3R,4S配置中立体选择性地氧化维里迪卡丁.
- 单模NRPS AplJ催化了一种罕见的分子内 Ester 键的形成.
- 细胞染色体P450 AplF,化酶AplN和前转移酶AplE修改了人类酸部分.
- AplB证明了对维里迪卡类似物具有放松的基质特异性.
结论:
- 已经阐明了阿斯佩拉林的生物合成途径.
- AplB显示出作为合成新型维里迪卡衍生物的生物催化剂的潜力.
- 了解阿斯佩拉林生物合成,为开发新的鱼类病原体治疗开辟了道路.
相关概念视频
Alkylation of β-Diester Enolates: Malonic Ester Synthesis
3.4K
Malonic ester synthesis is a method to obtain α substituted carboxylic acids from ꞵ-diesters such as diethyl malonate and alkyl halides.
3.4K
Preparation of Epoxides
7.5K
Overview
Epoxides result from alkene oxidation, which can be achieved by a) air, b) peroxy acids, c) hypochlorous acids, and d) halohydrin cyclization.
Epoxidation with Peroxy Acids
Epoxidation of alkenes via oxidation with peroxy acids involves the conversion of a carbon–carbon double bond to an epoxide using the oxidizing agent meta-chloroperoxybenzoic acid, commonly known as MCPBA. Since the O–O bond of peroxy acids is very weak, the addition of electrophilic oxygen of...
Epoxides result from alkene oxidation, which can be achieved by a) air, b) peroxy acids, c) hypochlorous acids, and d) halohydrin cyclization.
Epoxidation with Peroxy Acids
Epoxidation of alkenes via oxidation with peroxy acids involves the conversion of a carbon–carbon double bond to an epoxide using the oxidizing agent meta-chloroperoxybenzoic acid, commonly known as MCPBA. Since the O–O bond of peroxy acids is very weak, the addition of electrophilic oxygen of...
7.5K
Stereochemical Effects of Enolization
2.0K
The chiral α-carbon of the carbonyl compound is the stereocenter of the molecule. As shown in the figure below, when such a carbonyl compound undergoes racemization under an acidic or basic condition, an achiral enol is formed.
2.0K
Alkylation of β-Ketoester Enolates: Acetoacetic Ester Synthesis
3.3K
Acetoacetic ester synthesis is a method to obtain ketones from alkyl halides and β-keto esters. The reaction occurs in the presence of an alkoxide base that abstracts the acidic proton of the β-keto esters. The step results in an enolate ion which is doubly stabilized. The enolate then reacts with an alkyl halide via the SN2 process to produce an alkylated ester intermediate with a new C–C bond. The hydrolysis of the intermediate, followed by acidification, results in an...
3.3K
Stereoisomerism of Cyclic Compounds
8.7K
In this lesson, we delve into the role of ring conformation and its stability, which determines the spatial arrangement and, consequently, the molecular symmetry and stereoisomerism of cyclic compounds. 1,2-Dimethylcyclohexane is used as a case study to evaluate the possible number of stereoisomers. Here, given the multiple (n = 2) chiral centers, there are 2n = 4 possible configurations that lack a plane of symmetry, as the ring skeleton exists in a non-planar chair conformation. In addition,...
8.7K
Diels–Alder Reaction Forming Bridged Bicyclic Products: Stereochemistry
4.6K
Diels–Alder reactions between cyclic dienes locked in an s-cis configuration and dienophiles yield bridged bicyclic products.
4.6K

![Cercosporin-Photocatalyzed [4+1]- and [4+2]-Annulations of Azoalkenes Under Mild Conditions](/_next/image?url=https%3A%2F%2Fcloudfront.jove.com%2FCDNSource%2Fteasers%2F60786.jpg&w=3840&q=50)