农业土壤中的环境持久微生物污染:具有致病性和抗生素耐药性的高风险
Lin Zhu1, Yongchang Xu2, Jingpeng Li1
1International Science and Technology Cooperation Platform for Low-Carbon Recycling of Waste and Green Development & Zhejiang Provincial Key Laboratory of Solid Waste Treatment and Recycling, School of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou 310012, China.
环境持久性微生物携带毒性因子基因和最后的抗生素耐药性基因,造成严重的健康风险. 这些基因很容易在等离子体上传播,这突显了对综合"一健康"战略的需求.
科学领域:
- 环境微生物学环境微生物学
- 抗微生物耐药性 抗微生物耐药性
- 病原体生态学 病原体生态
背景情况:
- 环境中持续的微生物污染是常见的,但人们对其了解甚少.
- 在人类细菌病原体 (HBPs) 中,毒性因子基因 (VFGs) 和最后抗生素耐药性基因 (LARGs) 的同时出现引起了生态和健康方面的担忧.
研究的目的:
- 调查农业土壤中LARG寄存HBPs的病原性和抗生素耐药性.
- 了解VFG和LARG共同定位和传播的遗传机制和生态影响.
主要方法:
- 定量PCR (qPCR) 用于确定土壤中VFG和LARG的丰度.
- 隔离和表征LARG寄存的HBPs.
- 使用Galleria mellonella进行致病性测试.
- 等离子体分析以评估健康成本和遗传安排.
主要成果:
- 农业土壤中VFG和LARG的高分布 (高达4.7 × 10^7基因拷贝/g土壤).
- 大多数孤立的LARG携带的HBPs在G. mellonella中显示100%的致死率.
- 携带LARG的等离子体表现出较低的适应性成本,这表明宿主适应性很高.
- 有证据表明,等离子体融合和基因安排有助于稳定的水平基因转移.
结论:
- 由于LARG/VFG相关基因的稳定和广泛存在,环境持久的微生物污染带来了高风险.
- 这些发现强调了在环境微生物污染的"One Health"框架内考虑表型-基因型-利基互动的重要性.
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