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Direct-Acting Cholinergic Agonists: Pharmacokinetics

Direct-acting cholinergic agonists, such as synthetic choline esters and naturally occurring alkaloids, exert their effects by enhancing the actions of acetylcholine and stimulating the parasympathetic nervous system. Synthetic choline esters share structural similarities with acetylcholine. For example, they have a positively charged quaternary ammonium or onium group, contributing to their hydrophilic characteristics. As a result, they are poorly absorbed in the body through oral...
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Waste Water Derived Electroactive Microbial Biofilms: Growth, Maintenance, and Basic Characterization
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以胆为基础的深层性溶剂进行度调节,可诱导Bacillus subtilis生物膜中的电活性.

Neda Eghtesadi1, Kayode Olaifa1, Tri T Pham2

  • 1Department of Chemical and Materials Engineering, School of Engineering and Digital, Sciences, Nazarbayev University, 53 Kabanbay Batyr Avenue, Astana 01000, Kazakhstan.

Enzyme and microbial technology
|July 26, 2024
PubMed
概括

用胆化物和D-sorbitol深溶解剂处理的Bacillus subtilis生物膜显示在盐应激下增强了电活性和生物膜的产生,改善了微生物电化学技术的应用.

关键词:
这种细菌是 Bacillus subtilis.生物膜是一种生物膜.深层欧性溶剂 深层欧性溶剂透应激压力 透应激压力微弱的电气器

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科学领域:

  • 微生物生物技术 微生物生物技术
  • 生物电化学系统 生物电化学系统
  • 合成生物学 合成生物学

背景情况:

  • 细菌细菌 (Bacillus subtilis) 是一种格拉姆阳性细菌,是生物技术中的一个关键模型生物体,在生物膜中表现出较弱的电活性.
  • 在B. subtilis生物膜中增强细胞外电子转移 (EET) 对推进微生物电化学技术 (MET) 和电发酵至关重要.
  • 以前的研究表明,胆化物 (ChCl) 增强了B. subtilis.中的电流输出和生物膜形成.

研究的目的:

  • 为了研究D-sorbitol/胆化物 (ChCl) 深层欧溶剂 (DESs) 对B. subtilis在盐应力下的电活动的度调节和营养作用.
  • 优化B. subtilis中增强生物膜生产和细胞外电子转移的条件,用于生物技术应用.

主要方法:

  • 培养Bacillus subtilis在低碳的顿酵母提取物介质中,并添加盐来诱导透应激.
  • 添加D-sorbitol/胆化物 (ChCl) 深溶解剂 (1:1mol/mol),以评估它们对生物膜电活性的影响.
  • 在不同盐度和DES处理下分析度调节作用和生物膜特性.

主要成果:

  • 胆化物 (ChCl) 和D-sorbitol有效地减轻了由NaH2PO4和KH2PO4盐添加引起的透应激.
  • ChCl和D-sorbitol的组合显著增加了B. subtilis.生物膜的产生.
  • 观察到电活性增加,这可能是由于ChCl的奥斯摩保护作用和电活性外聚合物质的诱导.

结论:

  • 胆化物和D-sorbitol充当了氧化保护剂,减轻了盐应激,增强了Bacillus subtilis中的生物膜形成和电活性.
  • 在高离子强度介质中使用含有ChCl的DES是一种有前途的策略,用于改进基于生物膜的电发酵过程.
  • 这种方法为生物技术应用提供了显著的好处,特别是在高价值代谢物的生物生产中.