以胆为基础的深层性溶剂进行度调节,可诱导Bacillus subtilis生物膜中的电活性
Neda Eghtesadi1, Kayode Olaifa1, Tri T Pham2
1Department of Chemical and Materials Engineering, School of Engineering and Digital, Sciences, Nazarbayev University, 53 Kabanbay Batyr Avenue, Astana 01000, Kazakhstan.
Enzyme and microbial technology
|July 26, 2024
概括
用胆化物和D-sorbitol深溶解剂处理的Bacillus subtilis生物膜显示在盐应激下增强了电活性和生物膜的产生,改善了微生物电化学技术的应用.
科学领域:
- 微生物生物技术 微生物生物技术
- 生物电化学系统 生物电化学系统
- 合成生物学 合成生物学
背景情况:
- 细菌细菌 (Bacillus subtilis) 是一种格拉姆阳性细菌,是生物技术中的一个关键模型生物体,在生物膜中表现出较弱的电活性.
- 在B. subtilis生物膜中增强细胞外电子转移 (EET) 对推进微生物电化学技术 (MET) 和电发酵至关重要.
- 以前的研究表明,胆化物 (ChCl) 增强了B. subtilis.中的电流输出和生物膜形成.
研究的目的:
- 为了研究D-sorbitol/胆化物 (ChCl) 深层欧溶剂 (DESs) 对B. subtilis在盐应力下的电活动的度调节和营养作用.
- 优化B. subtilis中增强生物膜生产和细胞外电子转移的条件,用于生物技术应用.
主要方法:
- 培养Bacillus subtilis在低碳的顿酵母提取物介质中,并添加盐来诱导透应激.
- 添加D-sorbitol/胆化物 (ChCl) 深溶解剂 (1:1mol/mol),以评估它们对生物膜电活性的影响.
- 在不同盐度和DES处理下分析度调节作用和生物膜特性.
主要成果:
- 胆化物 (ChCl) 和D-sorbitol有效地减轻了由NaH2PO4和KH2PO4盐添加引起的透应激.
- ChCl和D-sorbitol的组合显著增加了B. subtilis.生物膜的产生.
- 观察到电活性增加,这可能是由于ChCl的奥斯摩保护作用和电活性外聚合物质的诱导.
结论:
- 胆化物和D-sorbitol充当了氧化保护剂,减轻了盐应激,增强了Bacillus subtilis中的生物膜形成和电活性.
- 在高离子强度介质中使用含有ChCl的DES是一种有前途的策略,用于改进基于生物膜的电发酵过程.
- 这种方法为生物技术应用提供了显著的好处,特别是在高价值代谢物的生物生产中.
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