探索蛋白质结构适应和多相互作用:在热和发芽条件下对豆豆和全谷物的消化能力的影响
Minakshi Dutta1, R Dineshkumar1, C R Nagesh1
1Division of Biochemistry, ICAR-Indian Agricultural Research Institute, New Delhi 110012, India.
Food chemistry
|July 26, 2024
概括
用热处理处理豆的加工,特别是自封,显著提高了它的蛋白质消化能力和营养质量. 这使豆类成为健康食品开发的更好的成分.
科学领域:
- 食品科学 食品科学 食品科学
- 营养生物化学 营养生物化学
- 农业科学 农业科学
背景情况:
- 豆是一种富含蛋白质的豆类,但其蛋白质消化能力 (PD) 受到诸如多之类的反营养因子 (ANF) 的限制.
- 处理方法对于克服这些局限性和提高豆的营养价值至关重要.
研究的目的:
- 评估各种热处理和发芽对豆蛋白质消化能力的影响.
- 分析处理后ANF含量和蛋白质结构性质的变化.
- 为了比较不同豆基因型的不同方法的疗效,具有不同的多醇水平.
主要方法:
- 在两种豆基因型 (高和低聚) 上研究了热处理 (,热水,自封,红外线) 和发芽.
- 在体外评估蛋白质消化能力和必需氨基酸含量.
- 分析了蛋白质的结构性质,包括二次结构变化 (β-转变) 和与粉和多的相互作用.
主要成果:
- 所有治疗都改善了体外PD和必需氨基酸含量.
- 自闭包体显示出最大的PD增加 (90.4%在ICP-1452,84.32%在普萨阿哈尔2018-4).
- 治疗导致β转变的增加,蛋白质:粉和蛋白质:多相互作用的减少,以及储存蛋白质的分解.
结论:
- 热处理,特别是自闭塞,有效地提高了豆蛋白质的消化能力.
- 这些方法改善了蛋白质的结构性质,并减少了ANF干扰.
- 增强的豆蛋白可以成为开发营养丰富的食品的宝贵成分.
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