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双M通过破坏细胞骨架架构和细胞循环过程,在体外抑制小鼠卵细胞成熟

Huilei Chen¹, Yang Liu², Yue Huang²

¹Reproductive Medicine Center, Department of Obstetrics and Gynecology, The First Affiliated Hospital of Anhui Medical University, No.218 Jixi Road, Hefei 230022, China; NHC Key Laboratory of Study on Abnormal Gametes and Reproductive Tract, Anhui Medical University, No.81 Meishan Road, Hefei 230032, China; Key Laboratory of Population Health Across Life Cycle, Anhui Medical University, Ministry of Education of the People's Republic of China, No.81 Meishan Road, Hefei 230032, China; Reproductive Medicine Center, Department of Obstetrics and Gynecology, The First Affiliated Hospital of Bengbu Medical University, No.287 Changhuai Road, Bengbu 233000, China.

Reproductive toxicology (Elmsford, N.Y.)

|July 26, 2024

概括

双M (BPM) 暴露损害了小鼠卵细胞的成熟,因为它破坏了半变异,组装和DNA完整性. 这种化学物质是双A (BPA) 的替代品,对生殖健康构成风险.

科学领域:

生殖生物学生殖生物学
毒理学毒理学毒理学
细胞生物学细胞生物学

背景情况:

双M (BPM) 是工业上使用的BPA替代品.
BPM会对健康产生有害影响.
它对卵细胞成熟的影响需要研究.

研究的目的:

评估BPM对小鼠卵细胞成熟的体外影响.
阐明BPM诱导的卵细胞功能障碍背后的分子机制.

主要方法:

在实验室中成熟的小鼠卵细胞.
介质性进展标志物的评估 (GVBD,PBE).
分析螺旋组装,染色体对齐,蛋白质降解 (securin,cyclin B1),微管体动力学 (α-tubulin乙化),actin组装,DNA损伤和基因组修饰.

主要成果:

暴露于BPM抑制了生殖囊泡的分解和极体挤出率.
通过激活螺旋组装检查点,BPM引起了介质停止.
BPM破坏了螺旋组装,染色体对齐,微管稳定性和动蛋白组装,同时增加了DNA损伤和改变了基因组修饰.

结论:

暴露于BPM显著损害了小鼠卵细胞的质量.
通过多个分子破坏,BPM抑制了介质成熟.
在生殖健康方面,BPM不是BPA的安全替代品.

关键词:

双M M 双M 是一种细胞骨架细胞骨架介质变化 (Meiosis) 是一个过程.卵细胞是卵细胞中的一个.

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