来自Aspergillus spectabilis的十个环-B芳香性埃尔戈斯特醇.
Mengsha Wei1, Weiguang Sun1, Linlin Liu1
1Hubei Key Laboratory of Natural Medicinal Chemistry and Resource Evaluation, School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Chinese journal of natural medicines
|July 26, 2024
概括
从Aspergillus spectabilis中分离出了10种新的芳香性埃尔戈斯特醇. 一种化合物,欧戈斯特醇8,通过减少细胞亡和维护线粒体功能,显示出显著的神经保护作用.
科学领域:
- 自然产品化学 自然产品化学
- 药用化学 医学化学
- 菌类学 菌类学是指菌类学.
背景情况:
- 阿斯伯吉卢斯种类是生物活性天然产品的丰富来源.
- 具有独特结构特性的ergosterols因其生物活性而引起人们的兴趣.
- 对真菌代谢物的探索可以导致发现新型治疗剂.
研究的目的:
- 从Aspergillus spectabilis中分离和描述新型的埃尔戈斯特.
- 为了研究这些新发现的化合物的神经保护潜力.
主要方法:
- 使用色谱技术分离和净化化合物.
- 使用高分辨率电喷射电离质谱法 (HR-ESI-MS) 和核磁共振 (NMR) 谱法进行结构阐明.
- 通过单晶X射线衍射和电子圆二元体 (ECD) 计算来确定绝对配置.
- 通过使用谷氨酸诱导的SH-SY5Y细胞模型进行神经保护活性的体外评估.
主要成果:
- 鉴定出了十种带有芳香B环的ergosterols,Spectasterols F-O,它们的成分.
- 化合物4呈现出不寻常的C23厄戈斯特醇结构,侧链被截断.
- 化合物8显示显著的剂量依赖的神经保护作用,减少细胞亡和增强线粒体功能在SH-SY5Y细胞.
结论:
- Aspergillus spectabilis 生产了一系列多样化的芳香性埃尔戈斯特醇.
- 化合物8是开发神经保护疗法的有希望的化合物.
- 对化合物8的作用机制进行进一步研究是有必要的.
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