一个超稳定的,低盐适应蛋白酶从halophilic archaeon与潜在的应用在盐发酵食品
Jing Hou1, Qing-Ke Zhang1, Ruo-Yao Zhang1
1School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Jingkou District, Zhenjiang 212013, Jiangsu, People's Republic of China.
Food research international (Ottawa, Ont.)
|July 26, 2024
概括
一个新的盐耐受性蛋白酶,HlyAΔCTE,是从素A.开发出来的. 这种酶表现出异常的稳定性和增强的活性,对盐发酵食品工业显示出很大的前景.
科学领域:
- 生物化学 生物化学
- 酶学 是一种酶学.
- 食品科学 食品科学 食品科学
背景情况:
- 耐盐蛋白酶是盐发酵食品工业的关键生物催化剂.
- 素A (HlyA) 是一种来自Halococcus salifodinae的低盐适应素.
- 对HlyA的C端延伸 (CTE) 的自动切割可以产生更稳定的变体.
研究的目的:
- 为了研究HlyA (HlyAΔCTE) 的自裂产品的特性.
- 在各种条件下评估HlyAΔCTE的稳定性和活性.
- 评估HlyAΔCTE作为鱼发酵中的生物催化剂的潜力.
主要方法:
- 通过操纵温度和NaCl度,HlyA受到自动切割.
- 在各种温度,NaCl度和pH值范围内测试了HlyAΔCTE的稳定性.
- 评估了酶动力学 (Vmax) 和鱼发酵中的性能.
主要成果:
- HlyAΔCTE通过HlyA.的自动切割成功产生.
- 在 -20-60 °C,0.5-4 M NaCl和pH 6.0-10.0之间,HlyAΔCTE在72小时内表现出了显著的稳定性.
- HlyAΔCTE在50-75°C,0.5-2.5M NaCl和pH 6.5-8.0处表现出最佳活性,其Vmax比HlyA.更高.
- 在发酵过程中,HlyAΔCTE显著改善了鱼蛋白水解.
结论:
- 据报道,HlyAΔCTE是第一个具有异常稳定性和独特活性配置文件的haloysin.
- 在盐发酵食品工业中,HlyAΔCTE显示出作为强大的生物催化剂来增强蛋白质水解的显著潜力.
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