在新鲜切割的生菜生产链中形成生物膜的Bacillus cereus群体的潜在风险
Kye-Hwan Byun1, Miseon Kang2, Min Seon Koo2
1Food Safety and Distribution Research Group, Korea Food Research Institute, Wanju, Jeollabuk-do 55365, Republic of Korea; Technology Innovation Research Division, Hygienic Safety and Materials Research Group, World Institute of Kimchi, Gwangju 61755, Republic of Korea.
Food research international (Ottawa, Ont.)
|July 26, 2024
概括
在新鲜切割的生菜上发现了Bacillus cereus和Bacillus thuringiensis. 大多数隔离物对常见的抗生素有耐药性,但对万科米辛敏感,具有显著的子和生物膜形成能力.
科学领域:
- 食品安全微生物学 食品安全微生物学
- 细菌遗传学和生理学
背景情况:
- 谷菌群细菌在自然界中很常见,可以引起食物传播疾病.
- 新鲜切割的产品,比如生菜,可以成为病原体传播的载体.
- 了解细菌特征对于食品生产中的风险评估至关重要.
研究的目的:
- 调查Bacillus cereus和Bacillus thuringiensis在新鲜切割的生菜生产中的流行率和风险潜力.
- 分析分离的基因型和表型特征,包括毒素基因,抗生素耐药性,化和生物膜形成.
- 为整个冷链的食品安全管理策略提供见解.
主要方法:
- 从新鲜切割的生菜生产链中收集和分离了131种Bacillus cereus群菌株.
- 对肠毒素和排泄毒素基因的基因型分析.
- 现型表征包括抗生素敏感性测试,分泌量测试和不同温度 (30°C和10°C) 的生物膜形成研究.
- 对焦激光扫描显微镜 (CLSM) 用于生物膜结构分析.
主要成果:
- 在Bacillus cereus中检测到肠毒素基因,但在任何隔离物中没有检测到排泄毒素基因.
- 所有分离物对万科米辛敏感,但对大多数β-乳酸抗菌剂有抗性.
- 隔离物在30°C时表现出子形成 (平均24.6%) 和生物膜形成.
- 在30°C强大的生物膜形成者在10°C时表现出增强的生物膜密度,在生物膜中观察到子.
结论:
- 在新鲜切割的生菜生产链中,Bacillus cereus和Bacillus thuringiensis是普遍存在的.
- 这些分离物具有诸如抗生素耐药性和强大的生物膜形成等有关特征,可能会影响食品安全.
- 调查结果强调需要有效的风险管理策略,特别是在冷链完整性和生物膜控制方面.
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