在卵细胞复合体预化影响下,卵细胞的转录形状的变化
Azam Govahi1, Sahar Eghbali2, Naser Elmi Ghiasi3
1Endometriosis Research Center, Iran University of Medical Sciences, Tehran, Iran.
Scientific reports
|July 26, 2024
概括
预化积卵细胞复合体 (COCs) 2小时改变了积卵细胞 (CC) 基因表达,增强了ATP生产至关重要的途径,并积极影响了卵细胞质量. 这有助于人们更好地理解辅助生殖中CC功能.
科学领域:
- 生殖生物学 生殖生物学
- 基因组学就是基因组学.
- 细胞的新陈代谢
背景情况:
- 累积细胞 (CCs) 在卵细胞成熟和质量方面发挥着至关重要的作用.
- 累积卵细胞复合体 (COCs) 的预化可能会影响CC功能和转录基因特征.
- 了解这些变化对于优化体外受精 (IVF) 结果至关重要.
研究的目的:
- 为了研究CCs在2小时的潜伏前期后的转录组变化.
- 确定预化对关键细胞通路的影响,这与卵细胞质量有关.
- 评估COC预化对辅助生殖技术的潜在好处.
主要方法:
- 从12名参与者中分离了140个累积细胞样本.
- 将样品分为两个组:即时剖析 (T0) 和2小时的预化 (T2).
- 使用RNA测序,用qRT-PCR进行确认,对CCs进行转录形状分析.
主要成果:
- 观察到基因表达的显著变化:与T0.0相比,T2组的17个基因下调和22个基因上调.
- 关键途径,包括ATP生产 (氧化酸化,电子运输链,OXPHOS系统),TNF-alpha信号传递和葡萄皮质受体途径在T2上调.
- 在T2组中发现TGF-β通路的下调.
结论:
- 2小时的预化期显著改变了CCs.的转录组特征.
- 这些变化涉及与细胞能量代谢和信号传递相关的关键路径.
- 观察到的转录组变化表明,COC预化可以积极影响卵细胞质量,可能提高试管婴儿成功率.
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