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Updated: Jun 19, 2025

Using Sniper-Cas9 to Minimize Off-target Effects of CRISPR-Cas9 Without the Loss of On-target Activity Via Directed Evolution
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Using Sniper-Cas9 to Minimize Off-target Effects of CRISPR-Cas9 Without the Loss of On-target Activity Via Directed Evolution

Published on: February 26, 2019

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使用分子建模开发小型Cas9杂交物种

Antoine Mangin1,2, Vincent Dion3,4, Georgina Menzies5

  • 1UK Dementia Research Institute at Cardiff University, Cardiff, CF24 4HQ, UK.

Scientific reports
|July 26, 2024
PubMed
概括

研究人员探索了较小的Cas9变体和混合体,以感染引起疾病的CAG/CTG重复,用于治疗神经肌肉疾病. 然而,这些工程系统未能实现基因编辑,这表明复杂的Cas9/sgRNA系统目前的in silico设计存在局限性.

关键词:
结合能量是有约束力的.这就是Cas9的情况.基因编辑 基因编辑分子动力学分子动力学

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