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相关概念视频

Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

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Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
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Microfluidic Device for the Separation of Non-Metastatic MCF-7 and Non-Tumor MCF-10A Breast Cancer Cells Using AC Dielectrophoresis
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使用确定性的横向位移微流体装置对差异化哺乳动物细胞进行分类.

Koji Matsuura1, Shingi Hashioka2, Koji Takata3

  • 1Department of Biosciences, Faculty of Life Science, Okayama University of Science, Okayama, Japan. kmatsuura@ous.ac.jp.

Analytical sciences : the international journal of the Japan Society for Analytical Chemistry
|July 26, 2024
PubMed
概括

本研究介绍了一种使用确定性横向位移 (DLD) 微流体装置的无标签细胞分离方法. 该系统有效地根据大小对差异化细胞进行分类,推进细胞分析和体外组织模型.

关键词:
脂肪细胞是一种脂肪细胞.细胞分类 细胞分类确定性的横向移位.不同化的细胞是不同的细胞.肌细胞 肌细胞

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科学领域:

  • 生物技术是生物技术.
  • 细胞生物学 细胞生物学
  • 微流体学 微流体学

背景情况:

  • 差异化和未差异化细胞的无标签分离对于体外细胞分析和临床应用至关重要.
  • 目前的方法通常需要细胞标签,限制它们的实用性.

研究的目的:

  • 开发和应用一个确定性的横移 (DLD) 微流体系统,用于无标签的细胞分离.
  • 根据大小差异对差异化细胞进行分类,以改进细胞分析和组织模型准备.

主要方法:

  • 一个确定性横向位移 (DLD) 微流体装置被开发用于被动细胞分离.
  • 压缩空气被用来驱动流体流动,控制原料和分类.
  • 该系统在C2C12单核肌细胞和多核肌管以及从小鼠胚胎纤维细胞 (MEF) 细胞中分化的脂肪细胞上进行了测试.

主要成果:

  • 多核菌管被有效地分类为更大的颗粒使用DLD微流体通道的临界直径 (Dc) 20μm.
  • 含有脂质滴滴的分化脂肪细胞,直径超过20微米,已成功分类.
  • 细胞大小被确定为区分细胞类型的可靠参数.

结论:

  • 开发的DLD微流体系统可以根据大小进行无标签的细胞分类.
  • 这项技术支持单细胞分析的进步和用于药物发现的体外组织模型的准备.