来自家禽生产链的高生物膜形成多药耐药沙门氏菌婴儿菌株
Laura Musa1, Valeria Toppi2, Valentina Stefanetti2,3
1Department of Veterinary Medicine and Animal Sciences, University of Milan, 26900 Lodi, Italy.
Antibiotics (Basel, Switzerland)
|July 27, 2024
概括
耐多药沙门氏菌 (Salmonella Infantis) 菌株在较低的温度下有效地形成生物膜,在肉养殖场环境中持久. 这凸显了在肉生产链中控制污染和确保食品安全的挑战.
科学领域:
- 微生物学 微生物学
- 食品安全 食品安全
- 环境科学 环境科学
背景情况:
- 沙门氏菌物种在表面形成生物膜的能力创造了持久的环境水库.
- 这种生物膜形成直接将环境污染与食品加工污染联系起来.
- 产生多药耐药 (MDR) 和扩展谱β-乳糖酶 (ESBL) 菌株的沙门氏菌为食品生产带来了重大关注.
研究的目的:
- 调查来自肉食物链的80种产生MDR和ESBL的沙门氏菌幼虫菌株的生物膜形成能力.
- 为了确定温度对生物膜形成能力的影响.
- 确定与这些菌株生物膜形成相关的遗传因素.
主要方法:
- 全基因组测序 (WGS) 和PCR被用于分析遗传元素.
- 在22°C和37°C时使用96孔聚烯板量化生物膜的形成.
- 在刚果红色 (CRA) 板上评估了粗和干燥的殖民地 (rdar) 形态.
主要成果:
- 所有测试的沙门氏菌Infantis菌株在22°C时产生生物膜,表现出rdar形态;只有一个菌株在37°C时形成生物膜.
- 58.75%的分离物体表现出强烈的生物膜产量,36.25%的中度,6.25%的弱度.
- 工作组证实了fimbriae生产基因 (fim和csg集群) 的存在;PCR验证了csgD,csgB和fimA在所有菌株中.
结论:
- 在较低的温度 (约22°C) 中,Salmonella Infantis生物膜的形成,特别是卷曲和纤维素的表达,是最佳的.
- 肉养殖场的环境温度 (18-22°C) 有利于沙门氏菌生物膜的形成和持久性.
- 这种持续性使生物安全,消毒协议复杂化,并在肉生产链中构成公共卫生风险.
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