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Updated: Jun 19, 2025

Protocols for Implementing an Escherichia coli Based TX-TL Cell-Free Expression System for Synthetic Biology
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在细菌表达系统中产生逆转录酶和DNA聚合酶

Kristína Hriňová1, Johana Dlapová1, Bohuš Kubala2

  • 1Department of Molecular Biology, Faculty of Natural Sciences, Comenius University in Bratislava, 84215 Bratislava, Slovakia.

Bioengineering (Basel, Switzerland)
|July 27, 2024
PubMed
概括

维布里奥纳特里基因是产生重组DNA放大和逆转录酶的有希望的宿主,为酶生产提供了适合于大肠杆菌的替代品. 这项研究证明了其产生可溶性Taq DNA聚合酶和突变M-MLV逆转录酶的潜力.

关键词:
DNA聚合酶是一种DNA聚合酶.埃舍里希亚大肠杆菌 (Escherichia coli) 是一个大肠杆菌.维布里奥纳特里根斯 (Vibrio natriegens) 是一种天然物质.细胞外生产的细胞外生产蛋白质生产 蛋白质生产反向转录酶的使用

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