Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Transcription Factors02:16

Transcription Factors

75.8K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
75.8K
General Transcription Factors01:30

General Transcription Factors

5.2K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
5.2K
Chromatin Immunoprecipitation- ChIP02:36

Chromatin Immunoprecipitation- ChIP

11.1K
Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...
11.1K
Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

6.4K
Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form...
6.4K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

A self-powered and reusable biocomputing security keypad lock system based on biofuel cells.

Chemistry (Weinheim an der Bergstrasse, Germany)·2010
Same author

Prevalence of nerve-vessel contact at cisternal segments of the oculomotor nerve in asymptomatic patients evaluated with magnetic resonance images.

Chinese medical journal·2010
Same author

Overexpression and characterization in Bacillus subtilis of a positionally nonspecific lipase from Proteus vulgaris.

Journal of industrial microbiology & biotechnology·2010
Same author

Strategies to minimize variability and bias associated with manual pipetting in ligand binding assays to assure data quality of protein therapeutic quantification.

Journal of pharmaceutical and biomedical analysis·2010
Same author

Medium- and long-chain triacylglycerols reduce body fat and blood triacylglycerols in hypertriacylglycerolemic, overweight but not obese, Chinese individuals.

Lipids·2010
Same author

[The clinical application of 320-slice Computed Tomography (CT) hepatic artery images in patients with liver transplantation].

Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology·2010

相关实验视频

Updated: Jun 19, 2025

Enhanced Yeast One-hybrid Screens To Identify Transcription Factor Binding To Human DNA Sequences
11:25

Enhanced Yeast One-hybrid Screens To Identify Transcription Factor Binding To Human DNA Sequences

Published on: February 11, 2019

7.9K

TFTF:一种基于R的整合工具,用于解码人类转录因子-目标相互作用.

Jin Wang1

  • 1School of Public Health, Suzhou Medical College, Soochow University, Suzhou 215123, China.

Biomolecules
|July 27, 2024
PubMed
概括

这项研究引入了一个新的R包,用于预测转录因子 (TF) 基因相互作用. 它集成了多种生物信息学工具,用于强大的基因调控网络分析.

科学领域:

  • 生物信息学是一种生物信息学.
  • 基因组学就是基因组学.
  • 系统生物学 系统生物学

背景情况:

  • 转录因子 (TFs) 调节基因表达,影响细胞和生物体的表型.
  • 识别TF目标基因相互作用对于理解分子通路和疾病至关重要.
  • 对于TF目标识别的传统实验方法通常是苛刻和劳动密集的.

研究的目的:

  • 开发一种新的R包和Web应用程序,用于预测转录因子-向基因关系.
  • 为分析基因调节机制和网络提供一个用户友好的平台.
  • 为了克服TF-目标相互作用发现的传统实验方法的局限性.

主要方法:

  • 整合多种生物信息学工具,以实现可靠的预测.
  • 合并生物数据库以提高预测准确度.
  • 利用基因表达相关性和全组织相关性分析来获得特定情境的见解.
  • 开发一个R包和Web应用程序,用于TF目标基因网络分析.

主要成果:

  • 一个新的R包和Web应用程序,用于预测TF-目标基因相互作用 (反之亦然).
  • 该应用程序集成了各种数据源和分析方法,用于全面的网络分析.
关键词:
闪闪发光的应用程序目标预测TF目标预测生物信息学是一种生物信息学.基因监管网络 基因监管网络转录因子是一种转录因子.

更多相关视频

Identifying Transcription Factor Olig2 Genomic Binding Sites in Acutely Purified PDGFRα+ Cells by Low-cell Chromatin Immunoprecipitation Sequencing Analysis
12:29

Identifying Transcription Factor Olig2 Genomic Binding Sites in Acutely Purified PDGFRα+ Cells by Low-cell Chromatin Immunoprecipitation Sequencing Analysis

Published on: April 16, 2018

9.2K
Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation
12:54

Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation

Published on: March 7, 2018

13.5K

相关实验视频

Last Updated: Jun 19, 2025

Enhanced Yeast One-hybrid Screens To Identify Transcription Factor Binding To Human DNA Sequences
11:25

Enhanced Yeast One-hybrid Screens To Identify Transcription Factor Binding To Human DNA Sequences

Published on: February 11, 2019

7.9K
Identifying Transcription Factor Olig2 Genomic Binding Sites in Acutely Purified PDGFRα+ Cells by Low-cell Chromatin Immunoprecipitation Sequencing Analysis
12:29

Identifying Transcription Factor Olig2 Genomic Binding Sites in Acutely Purified PDGFRα+ Cells by Low-cell Chromatin Immunoprecipitation Sequencing Analysis

Published on: April 16, 2018

9.2K
Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation
12:54

Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation

Published on: March 7, 2018

13.5K
  • 用户数据可以与现有资源集成,用于定制的网络探索.

    • 结论:

    • 开发的工具提供了一种强大而综合的方法来剖析复杂的基因调节机制.
    • 它是基因组学和分子生物学研究人员的宝贵资源.
    • 未来的扩展计划包括超出人类数据的额外物种.