在流体剪切应力下,乳腺癌细胞中干状表型的细胞外囊介导调节
Spenser R Brown1, Margaret E Radcliffe1, Joseph T Danner1
1Department of Chemical and Biological Engineering, The University of Alabama, Tuscaloosa, AL 35487, USA.
Biomolecules
|July 27, 2024
概括
液体剪切应力 (FSS) 增加了细胞外囊泡 (EV) 的产生和miR-21含量. 这些FSS诱导的EVs促进癌症干细胞特征和受体细胞中的增殖,突出了EVs.
科学领域:
- 癌症生物学 癌症生物学
- 细胞生物物理学 细胞生物物理学
- 细胞外囊泡 细胞外囊泡
背景情况:
- 循环瘤细胞 (CTC) 驱动癌症转移和死亡率.
- 在它们的微环境中,CTC会遇到流体剪切应力 (FSS).
- FSS可以诱导细胞外囊泡 (EV) 生产,调解细胞通信.
研究的目的:
- 研究生理学FSS对EV生产和内容的影响.
- 为了确定FSS调节的EV是否可以改变接受者癌细胞的特性.
- 探索特定的微RNA,如miR-21在FSS诱导的EV效应中的作用.
主要方法:
- 从MDA-MB-231癌细胞中分离EVs,使用直接流过,有或没有FSS.
- EV产量的表征,miRNA含量 (特别是miR-21) 和基因表达.
- 引入FSS衍生的EVs到MCF-7癌细胞,以评估表型变化.
主要成果:
- 在FSS条件下观察到显著增加的EV收益率.
- 根据FSS生产的电动汽车显示出高水平的miR-21.
- 暴露于FSS-EVs的受体MCF-7细胞表现出增加的干状基因表达,CD44+/CD24-癌症干细胞亚群,增殖和ATP生产.
结论:
- 生理学FSS直接影响电动汽车生产产量及其分子载荷.
- 在FSS相关的情形中,EV介导的miR-21转移起着至关重要的作用,例如癌症转移.
- 具有FSS条件的EV可以在癌细胞中促进更具侵略性的干状表型.
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