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在昆虫病原性线虫共生细菌中,III型分泌系统和鞭毛的功能和全球调节

Xiyin Huang¹, Chen Li¹, Ke Zhang¹

¹State Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Provincial Key Laboratory of Microbial Molecular Biology, College of Life Science, Hunan Normal University, Changsha 410081, China.

International journal of molecular sciences

|July 27, 2024

概括

旗驱动运动和生物膜的形成在虫病原性线虫共生细菌 (ENS). 鞭毛和III型分泌系统 (T3SS) 都有助于ENS的入侵和殖民,因为分泌系统之间有一个新发现的反循环.

科学领域:

微生物学微生物学
细菌病原体的产生
分子生物学分子生物学

背景情况:

第三类分泌系统 (T3SS) 携带细菌毒性因子,而鞭毛则提供运动性.
昆虫病原性线虫共生细菌 (ENS),如Xenorhabdus和Photorhabdus,在感染昆虫时利用这些系统.
对ENS中的T3SS和鞭毛功能进行比较研究是有限的.

研究的目的:

为了阐明T3SS和鞭毛在ENS感染中的不同和重叠的作用.
研究T3SS,鞭毛和VI型分泌系统 (T6SS) 之间的调控相互作用.

主要方法:

在 *Xenorhabdus stockiae* (*flhDC* 删除) 中进行鞭毛无活化.
在*Photorhabdus luminescens* (*sctV*删除) 中的T3SS无活化.
在*X. stockiae*中*P. luminescens* T3SS的异质表达.
蛋白质组分析和RT-PCR用于基因表达研究.

主要成果:

旗对ENS群动性和生物膜形成至关重要.
无论是T3SS还是鞭毛细胞,都会对ENS的入侵和宿主细胞的殖民产生影响.
在鞭毛/T3SS组件和T6SS之间存在负反循环;T3SS抑制鞭毛组件,而鞭毛蛋白促进T3SS组件.

结论:

旗和T3SS在ENS病变发生过程中发挥着互补的作用.
复杂的调节相互作用控制了细菌分泌系统的表达和组装.
T3SS表达影响细菌蛋白质合成,包括与核糖体相关的蛋白质.

关键词:

这就是T3SS.在T6SSSS中使用.昆虫病原性线虫共生细菌的细菌.旗小旗旗的意思

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