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使用费雷特签名加热EM工作流加速.

Pierre Nottelet1, Peter Van Blerkom1, Xiao-Ping Xu2

  • 1Department of Chemistry and Biochemistry, University of California Santa Barbara, Santa Barbara, CA 93106, USA.

International journal of molecular sciences
|July 27, 2024
PubMed
概括

费雷特签名方法现在可以在冷电子显微镜中确定首选的粒子方向,加速结构确定. 这种计算工具有助于优化复杂样本的数据收集和处理.

关键词:
渡轮签名的签名 渡轮签名分析工具是一种分析工具.低温电子显微镜的低温电子显微镜处理数据的数据处理.单个粒子分析结构生物学结构生物学结构的确定 结构的确定工作流的优化工作流的优化.

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Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
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