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强大的CXCL10/IP-10和CCL5/RANTES产物诱导的传染性脑炎病毒在人类大脑细胞中,尽管感染较弱

Veronika Prančlová^1,2, Václav Hönig^1,3, Marta Zemanová¹

¹Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, CZ-37005 Ceske Budejovice, Czech Republic.

International journal of molecular sciences

|July 27, 2024

概括

人类大脑周细胞通过释放关键信号分子来响应感染性脑炎病毒 (TBEV). 这项研究揭示了皮质细胞在TBEV脑部感染期间是化学激素的重要来源.

科学领域:

神经科学是一个神经科学.
病毒学病毒学
免疫学免疫学免疫学

背景情况:

传染性脑炎病毒 (TBEV) 感染中枢神经系统 (CNS),可能导致严重的神经问题.
神经血管单元对于中枢神经系统功能和TBEV神经侵袭至关重要.
人类大脑皮质细胞在TBEV感染中的特定作用尚不清楚.

研究的目的:

为了研究TBEV感染期间人类大脑初级皮质细胞的作用.
为了比较高毒性 (Hypr) 和轻毒性 (Neudoerfl) TBEV菌株对细胞周围细胞的影响.
分析受感染皮细胞释放的细胞因子,化学因子和生长因子.

主要方法:

主要人类大脑周细胞感染TBEVHypr和Neudoerfl菌株.
监测病毒复制动力学和细胞内病毒RNA.
使用Luminex测定和免疫光染色的细胞因子/化学因子的量化.

主要成果:

这两种TBEV菌株在细胞内同样复制,细胞内RNA在感染后3-6天达到峰值.
一小部分细胞细胞被感染,没有观察到细胞病变效应.
检测到IL-6,IL-15和IL-4,其中IP-10,RANTES和MCP-1是主要释放的化学激素.

结论:

人类大脑细胞支持TBEV复制,没有显著的细胞损伤.
在TBEV感染期间,细胞是关键化学激素 (IP-10,RANTES,MCP-1) 的重要来源.
这些发现突出显示了皮质细胞在脑中对TBEV的神经炎症反应中的作用.

关键词:

CCL5 CCL5 CCL5 CCL5 CCL5 CCL5 CCL5 CCL5 CCL5 CCL5 CCL5 CCL5 在CXCL10中,CXCL10是CXCL10中的一个.这是一种化学物质,chemokine.弗拉维病毒 (flavivirus) 是一种病毒.人类皮质细胞感染的感染感染.这是一种炎症炎症炎症炎症.感染性脑炎病毒

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