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基于W1/O/W2的双响应的"蛋盒"形状的微凝珠,用于针对结肠的协生菌剂的双重乳液
Xian He1, Yunyun Qin1, Haoyue Liu1
1Department of Nutrition and Food Hygiene, Center for Big Data and Population Health of IHM, School of Public Health, Anhui Medical University, Hefei 230032, China.
Foods (Basel, Switzerland)
|July 27, 2024
概括
这项研究开发了"蛋箱"微凝珠,以保护益生菌. 这种新型的输送系统显著改善了益生菌的生存率和消化道中受控的释放.
科学领域:
- 食品科学与技术 食品科学与技术
- 生物材料工程 生物材料工程
- 微生物学 微生物学
背景情况:
- 益生菌需要增强对环境因素的耐药性,才能有效提供.
- 现有的输送系统往往无法在整个消化道中保护益生菌.
- 需要新的封装策略来改善益生菌的生存和向释放.
研究的目的:
- 设计和制造新的微凝珠,以增强益生菌的传递.
- 调查设计的输送系统的结构特征和稳定性.
- 评估微凝珠在保护和释放益生菌方面的体外有效性.
主要方法:
- 制造 W1/O/W2 乳液,其稳定性由青果核素混合纳米颗粒 (ZAHP) 实现.
- 在聚-L-氨酸 (PLL) -酸盐-CaCl2 (Ca) 交联的凝层中封装乳液.
- 使用 ζ-电位和里埃变换红外光谱学对 ZAHPs 的表征.
- 通过光学和共聚焦激光扫描显微镜对W1/O/W2乳液的微结构可视化.
- 在体外结肠消化模拟以评估益生菌生存率.
主要成果:
- 具有2:1质量比的子-果果杂纳米粒子 (ZAHPs) 显示出出色的湿透性.
- 电静电相互作用和键被确定为ZAHP形成的关键力量.
- 成功可视化了W1/O/W2乳液的多腔结构.
- 与自由益生菌相比,微凝珠显著提高了益生菌的存活率 (75.11%).
- 封装中的益生菌显示出积极的储存稳定性,果果素既起乳化剂,又起益生菌作用.
结论:
- 开发的"蛋盒"形状的微凝珠有效地保护益生菌免受环境压力.
- 传递系统通过pH敏感和酶触发的机制提高了益生菌在消化道中的转移效率.
- 微凝珠使益生菌的空间时间控制释放成为可能,提高了它们的治疗潜力.
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