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相关概念视频

Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

5.6K
Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
5.6K
Flow Cytometry01:23

Flow Cytometry

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The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...
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Correction: Kang et al. Fluid Flow to Electricity: Capturing Flow-Induced Vibrations with Micro-Electromechanical-System-Based Piezoelectric Energy Harvester. <i>Micromachines</i> 2024, <i>15</i>, 581.

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相关实验视频

Updated: Jun 18, 2025

Microfluidic Buffer Exchange for Interference-free Micro/Nanoparticle Cell Engineering
10:27

Microfluidic Buffer Exchange for Interference-free Micro/Nanoparticle Cell Engineering

Published on: July 10, 2016

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使用光流体芯片进行无标签的连续细胞分类.

Yingjie Zhang1, Tao Zhang1, Xinchun Zhang1

  • 1Department of Mechanical Engineering, North China Electric Power University, Baoding 071003, China.

Micromachines
|July 27, 2024
PubMed
概括
此摘要是机器生成的。

这项研究介绍了一种用于非侵入性细胞分类的新型光纤针系统. 开发的光流体平台实现了高效率和细胞活力,而无需细胞标签,提供了具有成本效益的解决方案.

关键词:
这是一个T矩阵.细胞分类 细胞分类这就是为什么我们需要芯片,芯片芯片.高可行性 高可行性没有标签的无标签.光流体学是指光流体学.散射力是一种散射力.

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Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles
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Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles

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A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
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A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

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相关实验视频

Last Updated: Jun 18, 2025

Microfluidic Buffer Exchange for Interference-free Micro/Nanoparticle Cell Engineering
10:27

Microfluidic Buffer Exchange for Interference-free Micro/Nanoparticle Cell Engineering

Published on: July 10, 2016

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Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles
11:54

Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles

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A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
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A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

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科学领域:

  • 生物医学工程 生物医学工程
  • 光学是什么?光学是什么?光学是什么?
  • 细胞生物学 细胞生物学

背景情况:

  • 高效且非侵入性的细胞隔离是生物医学中的一个关键挑战.
  • 光纤子提供精确,非侵入性的细胞操纵,并与微流体系统集成得很好.

研究的目的:

  • 用光纤针来研究使用散射力进行细胞操纵.
  • 开发和验证一种用于连续细胞分类的新型光纤笔系统.

主要方法:

  • 用于细胞操纵的平端单模纤维.
  • 根据T矩阵模型推导出一个分散力公式.
  • 开发了一个光流体平台,集成光学子和微流体芯片,具有扩展的跨通道设计.

主要成果:

  • 成功实现了酵母细胞 (8-10微米) 和聚烯微球 (15-20微米) 的连续分离.
  • 达到高达86%的分类效率.
  • 在大约90%的分类酵母细胞中保持活力.

结论:

  • 开发的光纤针系统可实现高效,非侵入性和连续的细胞分类.
  • 这种无标签的系统为现有的细胞分类技术提供了具有成本效益的替代方案.
  • 光流体平台显示了生物医学应用的巨大潜力,需要精确的细胞操纵.