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通过比较16S rRNA测序与当地乳制品动物对希腊北部人乳微生物群的分析
Margaritis Tsifintaris1, Michail Sitmalidis1, Maria Tokamani1
1Department of Molecular Biology & Genetics, Democritus University of Thrace, 68100 Alexandroupolis, Greece.
Nutrients
|July 27, 2024
概括
人类和动物的牛奶共享一个核心微生物群,揭示了物种间保存的微生物群落. 这一发现对婴儿营养和乳制品安全有影响.
科学领域:
- 微生物学 微生物学
- 进行比较基因组学.
- 哺乳动物生物学 哺乳动物生物学
背景情况:
- 牛奶是一种重要的营养来源,其动态成分受到各种因素的影响.
- 牛奶的微生物群对其整体特性和健康影响起着至关重要的作用.
- 了解牛奶微生物群对于婴儿营养和乳制品安全至关重要.
研究的目的:
- 为了比较人类母乳 (乳酪和成熟) 中的微生物群落与成熟的山羊和牛奶中的微生物群落.
- 识别不同哺乳动物乳类型的共享微生物群落和流行属.
- 描述希腊北部人口中的牛奶微生物群.
主要方法:
- 使用16S rRNA元基因组测序来分析牛奶样本.
- 分析了来自五位母亲的十个母乳样本 (母乳和成熟).
- 分析了五个山羊奶和五个牛奶样本,以进行跨物种比较.
主要成果:
- 阿尔法多样性各异:在牛中适度,在山羊中高,在母乳中受限制.
- 贝塔多样性显示了物种之间的显著区别,基于存在/缺席和丰度进行聚类.
- 在所有物种中都发现了一种核心微生物群,主要是蛋白质菌,菌菌和动菌菌. 像Acinetobacter,Gemella和Sphingobium这样的特定属在人乳中更为丰富,而Pseudomonas和Atopostipes在动物乳中更为普遍.
结论:
- 哺乳动物的牛奶包含了特定物种和保存的微生物群落.
- 在人类,牛奶和山羊奶中存在一个共同的核心牛奶微生物组.
- 大奶粉和成熟人乳之间的保留差异突出了基本相似之处,这对婴儿营养和乳制品安全有影响.
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