AG1®,一种新型共生菌,在可可-2/THP1-BlueTM共培养模型中在炎症挑战后保持肠道屏障功能
Philip A Sapp1, Jeremy R Townsend1,2, Trevor O Kirby1
1Research, Nutrition, and Innovation, AG1, Carson City, NV 89701, USA.
Microorganisms
|July 27, 2024
概括
这项研究表明,在体外模型中,共生生物AG1可能通过在炎症挑战后维持体电阻 (TEER) 来保护胃肠道 (GI) 透性.
科学领域:
- 胃肠病学 胃肠病学
- 微生物学 微生物学
- 营养科学 营养科学
背景情况:
- 胃肠道 (GI) 透性对于身体活跃的个人和患有慢性疾病的人来说至关重要.
- 提高胃肠道屏障功能的营养策略具有显著的兴趣.
- 研究了一种新型协生菌,AG1,研究其对肠道透性的影响.
研究的目的:
- 在人体消化道模型中评估协生菌AG1对消化道透性的影响.
- 评估AG1在减轻肠道屏障炎症损伤方面的潜力.
- 探索AG1对胃肠道透性的影响背后的机制.
主要方法:
- 在实验室中使用了一种模拟胃,小肠和结肠的人类消化道模型.
- 在Caco-2/THP1-BlueTM共同培养模型中,评估了 GI 透性的体电阻 (TEER).
- 该模型在AG1或对照干预后使用脂多糖 (LPS) 进行了炎症挑战.
主要成果:
- 与对照组相比,AG1补充剂在炎症挑战后显著增加了TEER (p < 0.05).
- AG1保持了与负对照相比较的TEER水平,表明它对消化道屏障有保护作用.
- 在AG1和对照组之间没有观察到测量炎症标志物的显著差异.
结论:
- 实验室数据表明,AG1可能对肠道透性产生保护作用.
- AG1的前和益生菌成分可能有助于增强消化道屏障功能,可能通过短链脂肪酸 (SCFA) 生产.
- 需要进一步的研究来证实这些发现并阐明精确的机制.
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