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Updated: Jun 18, 2025

Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS
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Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS

Published on: July 11, 2016

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MALDI-TOF MS的应用,以识别和检测与牛乳腺炎相关的抗菌耐药性Streptococcus uberis

Tingrui Zhang1,2, Duangporn Pichpol3, Sukolrat Boonyayatra1,4

  • 1Department of Food Animal Clinic, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, Thailand.

Microorganisms
|July 27, 2024
PubMed
概括

矩阵辅助激光脱离离子化飞行时间质谱 (MALDI-TOF MS) 可以识别牛乳腺炎病原体Streptococcus uberis,并检测抗菌素耐药性 (AMR) 生物标志物. 这种蛋白质基的方法有助于诊断和管理乳牛中的AMR.

关键词:
马尔迪-托夫 (MALDI-TOF) 是一个国家元首.Streptococcus uberis 是一个细菌.抗微生物耐药性 抗微生物耐药性牛乳腺炎是一种牛乳腺炎.

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MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.
Matrix-assisted laser desorption ionization (MALDI) is a commonly...
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Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
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Matrix-assisted laser desorption ionization (MALDI) is a powerful analytical technique used in mass spectrometry. It enables the identification and characterization of various biomolecules, including proteins, peptides, nucleic acids, and carbohydrates. MALDI spectrometry is widely employed in biological and medical research, as well as in fields like pharmacology and biochemistry.
The analyte of interest, a biomolecule or a mixture of biomolecules, is mixed with a suitable matrix material. The...
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