相关实验视频

Updated: May 5, 2026

High-throughput Assay to Phenotype Salmonella enterica Typhimurium Association, Invasion, and Replication in Macrophages

High-throughput Assay to Phenotype Salmonella enterica Typhimurium Association, Invasion, and Replication in Macrophages

Published on: August 11, 2014

从子中分离出来的多抗药沙门氏菌的致病性Salmonella typhimurium

Yulin Xu^1,2, Zhitong Yu³, Shaopeng Wu³

¹Shandong Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan 250100, China.

|July 27, 2024

概括

在养场发现了沙门氏菌 (S. typhimurium) 菌株,其中一些菌株表现出多药耐药性和毒性基因. 幼感染导致生长减缓和器官损伤,突显了食物链的风险.

科学领域:

兽医微生物学兽医微生物学
食品安全食品安全
动物卫生动物健康

背景情况:

沙门氏菌 (Salmonella typhimurium,S. typhimurium) 是家禽中常见的一种血清型,造成了严重的经济损失.
沙门氏菌的抗生素耐药性是一个日益严重的全球性问题,因广泛使用抗生素而加剧.

研究的目的:

调查S. typhimurium在子养殖场的流行,耐药性和毒性.
为了评估选定S. typhimurium菌株在肉幼中的致病性.

主要方法:

从子胚胎和克洛亚克抹布中分离和识别S. typhimurium.
抗微生物敏感性测试和病毒性基因的检测.
用肉幼进行动物感染实验和组织学/免疫学分析.

主要成果:

隔离了八种S. typhimurium菌株;三种表现出多药耐药性 (MDR).
观察到关键病毒性基因 (invA,hilA,pefA,rck,sefa) 的高流行率.
在幼中感染22SD07菌株导致体重减轻,器官指数改变,肠道损伤和调节的细胞因子表达 (IL-10,TNF-α).

结论:

来自养殖场的S. typhimurium菌株具有药物耐药性和毒性特征.
由于S. typhimurium的致病性,通过食物链对人类健康构成潜在的风险.
持续监测和控制策略至关重要,以减轻S. typhimurium在子种群中的感染和传播.

关键词:

沙门氏菌的型号是沙门氏菌.动物实验动物实验具有多种耐药性的药物.病原性病原性病原性

更多相关视频

Characterizing Salmonella Typhimurium-induced Septic Peritonitis in Mice

Characterizing Salmonella Typhimurium-induced Septic Peritonitis in Mice

Published on: July 29, 2022

Automated Analysis of Intracellular Phenotypes of Salmonella Using ImageJ

Automated Analysis of Intracellular Phenotypes of Salmonella Using ImageJ

Published on: August 9, 2022

相关概念视频

Reservoir of Infection

Reservoir of Infection

Infectious diseases arise from intricate interactions between pathogens and their reservoirs. A reservoir of infection refers to the natural habitat where a pathogen lives, grows, and multiplies, serving as a continual source of infection. Reservoirs are broadly classified as either living or nonliving, and each plays a unique role in disease transmission, significantly influencing public health interventions and control strategies.Humans act as reservoirs for a wide array of pathogens,...

Bacterial Gastroenteritis

Bacterial Gastroenteritis

Bacterial gastroenteritis, characterized by diarrhea, abdominal cramps, and vomiting, is often caused by ingestion of contaminated food or water and is frequently associated with pathogenic Escherichia coli strains. These microbes exploit two principal mechanisms to inflict disease.Shiga toxin–producing E. coli, also referred to as STEC—notably O157:H7—release Shiga toxins that target ribosomes, blocking protein synthesis. The B subunit of the toxin binds the host glycolipid...

Clinical Significance of Antibiotic Resistance

Clinical Significance of Antibiotic Resistance

Methicillin-resistant Staphylococcus aureus (MRSA) presents a critical public health threat, arising from its capacity to resist β-lactam antibiotics due to acquisition of the mecA gene within the staphylococcal cassette chromosome mec (SCCmec). This gene encodes penicillin-binding protein 2a (PBP2a), which impairs binding efficacy of methicillin and other β-lactams. MRSA has evolved into distinct clonal lineages impacting humans and animals alike, reinforcing its significance within...