在乳制品农场新生小牛的Cryptosporidium感染
Michaela Kaduková1, Andrea Schreiberová1, Pavol Mudroň2
1Department of Epizootiology, Parasitology and Protection of One Health, University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, 040 01 Košice, Slovakia.
Microorganisms
|July 27, 2024
概括
分子鉴定揭示了斯洛伐克小牛中的Cryptosporidium parvum和Cryptosporidium ryanae. 牛犊是动物性传染病Cryptosporidium parvum的储存库,对公共卫生构成风险.
科学领域:
- 兽医寄生虫学 兽医寄生虫学
- 分子流行病学分子流行病学
- 动物传播疾病 动物传播疾病
背景情况:
- 克里普托斯波里迪亚 spp. 是显著的肠道原生虫寄生虫,在年轻牲畜中引起密码菌病.
- 牛犊容易受到Cryptosporidium感染,这可能会对经济和公共卫生产生影响.
- 早期识别和分子表征对于理解传播动态至关重要.
研究的目的:
- 在预先断奶的小牛中进行Cryptosporidium物种和亚型的分子鉴定.
- 确定斯洛伐克东部乳牛中密码菌病的流行率和发病率.
- 评估已识别的Cryptosporidium分离物的动物感染潜力.
主要方法:
- 从25头小牛每周从出生到5周收集便样本.
- 使用Ziehl-Neelsen染色和ELISA进行查,以检测Cryptosporidium抗原.
- 通过PCR进行分子表征,并对18SrRNA和gp60基因进行测序.
主要成果:
- 在第2周,ELISA检测到了最高的发病率 (91.6%);Ziehl-Neelsen在第2周显示了62.5%的发病率.
- 对29个分离物的分子分析确定了Cryptosporidium parvum和Cryptosporidium ryanae.
- 25个分离物是C. parvum,具有gp60基因型IIaA17G1R1;小牛是这种动物感染物种的储库.
结论:
- 斯洛伐克东部的小牛感染了C. parvum和C. ryanae.
- 牛犊中的C. parvum通过直接接触和环境暴露对人类构成重大动物感染风险.
- 有效的农场生物安全和公共卫生措施对于减轻传播至关重要.
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