提取的牙气膜的微生物组成在狗的晚期牙周炎
Laura Šakarnytė1, Raimundas Mockeliūnas1, Rita Šiugždinienė1
1Microbiology and Virology Institute, Lithuanian University of Health Sciences, 44307 Kaunas, Lithuania.
Microorganisms
|July 27, 2024
概括
狗的严重牙周炎含有可转移到人类的细菌,包括多抗性菌株. 从一个健康的角度来看,了解狗的口腔微生物群对于动物和人类的健康至关重要.
科学领域:
- 兽医微生物学 兽医微生物学
- 基因组学就是基因组学.
- 一个健康,一个健康.
背景情况:
- 牙周炎是一种严重的牙感染,影响狗,导致组织和骨损伤.
- 狗的口腔微生物群在牙周病中发挥着作用,这对公众健康有影响.
研究的目的:
- 为了研究严重牙周炎的狗的口腔微生物群.
- 为了将传统的培养方法与用于识别细菌的元基因组测序进行比较.
- 评估犬类牙周病原体的抗微生物耐药性.
主要方法:
- 利用传统的微生物学涂层和猎枪甲基因组测序在从狗身上提取的牙气泡上.
- 进行了细菌学测试以进行兽医诊断.
- Illumina HiSeq平台被用于元基因组测序.
主要成果:
- 基因组测序确定了*Porphyromonas gulae*,*Prevotella* spp. 和*Tannerella forsythia*作为流行物种.
- 传统方法经常分离出 *Pasteurella*, *Streptococcus* 和 *Neisseria*,仅占总微生物群的 1.6%.
- 观察到对多种抗生素的抗微生物耐药性,包括安培和多西环素,还检测到氨基糖化物和类的耐药性基因.
结论:
- 狗牙周炎涉及复杂的微生物群,在培养依赖和培养独立方法之间存在显著差异.
- 患有牙周感染的狗可以携带动物感染性和多药耐药细菌.
- 有效的治疗和预防犬牙周病对于一个健康方法来减轻对人类健康的风险至关重要.
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