从巴西肉中分离和鉴定大肠杆菌 (Escherichia coli)
Giulia Von Tönnemann Pilati1, Gleidson Biasi Carvalho Salles1,2, Beatriz Pereira Savi1
1Laboratory of Applied Virology, Department of Microbiology, Immunology and Parasitology, Federal University of Santa Catarina, Florianópolis 88040-900, Brazil.
Microorganisms
|July 27, 2024
概括
禽类致病性大肠杆菌 (APEC) 在巴西家禽中普遍存在,造成了严重的经济损失. 这项研究发现,群中APEC和有关抗菌素耐药性的高发病率.
科学领域:
- 兽医微生物学 兽医微生物学
- 禽类的健康 禽类的健康
- 抗微生物耐药性 抗微生物耐药性
背景情况:
- 禽类致病性大肠杆菌 (APEC) 引起大肠杆菌病,这是工业家禽养殖的主要疾病.
- 亚太经合组织导致高发病率,死亡率和尸体的谴责,导致巨大的经济损失.
- 了解巴西的APEC流行率和特征对于疾病控制至关重要.
研究的目的:
- 从巴西的尸检中分离和描述APEC.
- 确定APEC菌株的流行率并评估它们的抗菌素耐药性概况.
- 通过全基因组测序,在巴西APEC分离物中识别病毒性和耐药性基因.
主要方法:
- 从六个巴西州的100批肉中收集样本.
- 在MacConkey agar上隔离大肠杆菌,然后对APEC预测基因进行PCR.
- 抗微生物敏感性测试和全基因组测序用于详细的特征.
主要成果:
- 在研究的巴西家禽群中发现APEC的流行率为58%.
- 分离物对多种类型的抗生素表现出显著的耐药性,包括氨基糖化物 (79.36%),硫胺 (74.6%),β-乳酸盐 (63.49%) 和四环素 (49.2%).
- 亚太经合组织菌株含有关键的毒性基因,与粘附,铁吸收,血清耐药性和毒素产生有关.
结论:
- APEC是巴西家禽中重要的病原体,具有广泛的抗菌素耐药性.
- 这些发现突显出需要在家禽养殖中改善生物安全和抗菌药物管理.
- 基因组表征揭示了巴西APEC分离物中关键毒性和耐药性决定因素的存在.
关键词:
巴西家禽是巴西的家禽.抗微生物耐药性 抗微生物耐药性抗微生物耐药性基因的抗微生物耐药性基因禽类致病性大肠杆菌 (Escherichia coli) 是一种致病性大肠杆菌.这是一种大肠杆菌菌病.更多相关视频
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