通过使用修改的多糖聚合物进行氨酸的输送:制备,表征和体外释放特性
Chenchen Zhang1,2, Yan Zhang3, Jiangfeng Song1,2,3,4
1Institute of Agro-Product Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.
Polymers
|July 27, 2024
概括
用酸修饰的多糖 (SA-BP) 聚合物有效封装黄蛋白,显著提高其生物可用性和吸收性. 这些新型载体在提供蛋白等疏水性营养素方面表现有前途.
科学领域:
- 材料科学 材料科学 材料科学
- 生物技术是生物技术.
- 营养科学 营养科学
背景情况:
- 氨酸是一种重要的胡卜素,由于其疏水性质,具有较差的生物利用性.
- 开发有效的输送系统对于增强黄蛋白吸收和治疗潜力至关重要.
研究的目的:
- 为了制造用于蛋白加载的新型自组装聚合物,用酸修饰的鱼多糖化物 (SA-BP).
- 在模拟的胃肠道条件下,研究SA-BP聚合物中封装的黄蛋白的释放和吸收特性.
主要方法:
- 合成SA-BP与不同程度的替代 (DS) 蛋白封装.
- 使用颗粒大小分析,XRD,FT-IR和FS的SA-BP聚合物的表征.
- 在实验室中评估模拟胃和肠液中黄蛋白释放的情况.
- 在Caco-2细胞模型中评估黄蛋白运输和吸收机制.
主要成果:
- 在SA-BP聚合物 (227-341 nm) 中,黄蛋白的封装效率超过了90%.
- XRD证实了蛋白在聚合物中的非晶状状态;FT-IR/FS表明在疏水域的局部化.
- 与未经修改的样本相比,氨酸的生物可用性增加了4.36倍.
- 聚合物表现出胃稳定性和增强的肠道释放,吸收主要通过洞穴依赖和克拉依赖的内细胞分裂.
结论:
- SA-BP聚合物是疏水性黄蛋白的有效载体,提高了其稳定性和生物可用性.
- 涉及的内分细胞形成途径表明有高效的细胞吸收机制.
- SA-BP聚合物具有开发先进的黄蛋白输送系统的巨大潜力.
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