通过从Aspergillus nidulans中重新组合的丁酶进行的多乳酸降解
Eric Alvarado1, Rafael Castro1, José Augusto Castro-Rodríguez1
1Departamento de Alimentos y Biotecnología, Facultad de Química, UNAM, Mexico City 04510, Mexico.
Polymers
|July 27, 2024
概括
来自Aspergillus nidulans的三个丁酶可以降解聚乳酸 (PLA) 包装. ANCUT 1显示乳酸释放率最高,为PLA残留物降解提供了绿色解决方案.
科学领域:
- 生物技术是生物技术.
- 聚合物科学 聚合物科学
- 酶学 是一种酶学.
背景情况:
- 聚乳酸 (PLA) 是一种可生物降解的聚合物,广泛用于包装.
- 聚烯残留物的积累需要环保的降解方法.
- 酶降解是一个有希望的绿色替代品.
研究的目的:
- 为了评估来自阿斯伯吉卢斯尼杜兰斯的三种割分析酶对PLA降解.
- 为了比较它们对蛋白酶K的疗效.
- 为了优化增强PLA水解的条件.
主要方法:
- 选三种阿斯伯吉路斯尼杜兰斯的皮质酶,以检测PLA-化活性.
- 释放的乳酸的量化.
- 测量聚合物减重的方法.
- 对聚L-乳酸和聚D-乳酸的酶活性测定.
主要成果:
- 这三种丁酶都对PLA表现出活性.
- ANCUT 1 释放的乳酸量最高 (25.86 mM).
- 优化条件使乳酸释放翻了一番,导致45.96%的体重减轻.
- ANCUT 1对多L和多D乳酸反体都表现出活性.
结论:
- 阿斯伯吉illus nidulans 丁酶,特别是 ANCUT 1,显示出快速降解PLA残留物的显著潜力.
- ANCUT 1提供了一个可行的,绿色的酶解决方案,用于管理PLA包装废物.
- 进一步优化可以提高工业应用的降解效率.
关键词:
亚斯珀吉勒斯尼杜兰斯 (Aspergillus nidulans) 是一种植物.关键词: 关键词: 关键词: 关键词: 关键词: 关键词: 关键词:乳酸乳酸是什么意思 乳酸是什么意思聚 (乳酸) 降解降解的过程.更多相关视频
16:33Methods for Facilitating Microbial Growth on Pulp Mill Waste Streams and Characterization of the Biodegradation Potential of Cultured Microbes
Published on: December 12, 2013
9.5K
08:21Isolation and Screening from Soil Biodiversity for Fungi Involved in the Degradation of Recalcitrant Materials
Published on: May 16, 2022
4.7K
相关概念视频
Production of Organic Acids
Lactic acid, an important organic acid extensively applied in food, pharmaceutical, and biodegradable polymer industries, is primarily produced via microbial fermentation. This method is favored over chemical synthesis due to its environmental sustainability and capacity for enantiomerically pure product formation. Among various microbial processes, the fermentation of starch-based substrates stands out due to the abundance and renewability of raw materials like corn and potatoes.Hydrolysis of...
Bioplastics
Bioplastics derived from microbial processes present a sustainable alternative to conventional petroleum-based plastics. Among these, polyhydroxyalkanoates (PHAs), particularly polyhydroxybutyrates (PHBs), have emerged as prominent candidates due to their biodegradability and biocompatibility. These polymers are synthesized by a variety of bacteria, such as Cupriavidus necator and Pseudomonas putida, which naturally accumulate PHAs as intracellular carbon and energy reserves, especially under...
Microbial Bioremediation of Plastics
Polyethylene terephthalate (PET) is a synthetic polymer widely utilized in the packaging industry, particularly for bottles and containers. Due to its chemical stability and durability, PET accumulates in the environment, contributing significantly to plastic pollution. It comprises repeating units of terephthalic acid and ethylene glycol, resulting in a semi-crystalline structure that is resistant to natural degradation processes.A notable breakthrough in plastic biodegradation came with the...
