相关实验视频

Updated: Jun 18, 2025

Engineering Antiviral Agents via Surface Plasmon Resonance

Engineering Antiviral Agents via Surface Plasmon Resonance

Published on: June 14, 2022

一种基于的动态和有效的策略,以迅速解决新出现的SARS-CoV-2变种的关注问题

Michela Murdocca¹, Isabella Romeo^2,3, Gennaro Citro¹

¹Department of Biomedicine and Prevention, University of Rome Tor Vergata, Via Montpellier 1, 00133 Rome, Italy.

Pharmaceuticals (Basel, Switzerland)

|July 27, 2024

概括

一种新型的抑制剂,DPP4衍生,通过阻止病毒进入,有效地准SARS-CoV-2变体. 在疫苗开发过程中,这种提供了针对新兴变异的快速治疗策略.

科学领域:

病毒学病毒学
免疫学免疫学免疫学
药物发现药物发现药物发现

背景情况:

对SARS-CoV-2的基因组监测跟踪病毒遗传变化,以提供精确的公共卫生.
新出现的变种需要快速的治疗干预,以减轻大流行病的传播.
抑制SARS-CoV-2受体结合域 (RBD) 与细胞受体的相互作用是关键的治疗策略.

研究的目的:

开发针对新出现的SARS-CoV-2变种的基于的治疗策略.
设计一种针对病毒RBD和DPP4受体之间的相互作用的新型抑制剂.
为了评估设计的对SARS-CoV-2变体的疗效和结合亲和力.

主要方法:

基于二二酶4 (DPP4) 序列的抑制剂的合理设计.
在人体Calu-3细胞中对VSV* DG-Fluc伪病毒Omicron的的有效性进行了体外试验.
表面等离子体共振 (SPR) 确认和Omicron变异RBD之间的结合亲和力.

主要成果:

获得了一种强大的新型抑制剂,DPP4衍生.
由DPP4衍生而来的对向SARS-CoV-2变种表现出很高的亲和力.
在体外测试显示,Omicron变种感染能力减少了多达14%.
SPR证实了DPP4衍生的与Omicron变异RBD的结合.

结论:

DPP4衍生的代表了对抗SARS-CoV-2的有前途的内源性治疗剂.
这种提供了一个动态和迅速的策略,以抵消流行病早期的病毒变异.
可以在合成,稳定性和成本方面提供优势,以便快速开发治疗方法.

关键词:

其他RBD变体这就是SARS-CoV-2病毒.酸是一种酸.蛋白蛋白相互作用 (PPI) 是一种蛋白蛋白相互作用.伪病毒伪病毒表面的等离子体共振.

更多相关视频

A Fluorogenic Peptide Cleavage Assay to Screen for Proteolytic Activity: Applications for coronavirus spike protein activation

A Fluorogenic Peptide Cleavage Assay to Screen for Proteolytic Activity: Applications for coronavirus spike protein activation

Published on: January 9, 2019

A "Plug-And-Display" Nanoparticle Vaccine Platform Based on Outer Membrane Vesicles Displaying SARS-CoV-2 Receptor-Binding Domain

A "Plug-And-Display" Nanoparticle Vaccine Platform Based on Outer Membrane Vesicles Displaying SARS-CoV-2 Receptor-Binding Domain

Published on: July 25, 2022

相关概念视频

Conjugated Proteins

Conjugated Proteins

Simple proteins and protein complexes contain only amino acids. In contrast, many other proteins, called conjugated proteins, covalently bond with non-protein moieties.
Nucleoproteins are protein complexes that contain nucleic acids, categorized as deoxyribonucleoproteins (DNPs) or ribonucleoproteins (RNPs) respectively. The nucleosome is a typical example of a DNP where nuclear DNA is associated with histone proteins. The major antigen for the Covid-19 virus SARS-CoV is an RNP that is critical...

Leaky Scanning

Leaky Scanning

During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R...