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Equipments Used to Measure Body Temperature01:13

Equipments Used to Measure Body Temperature

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Body temperature can be assessed using various devices and measured in Celsius or Fahrenheit.
Glass-bulb Thermometer:
Glass-bulb thermometers are hollow glass tubes with a bulb tip containing liquid such as ethanol or mercury. Historically, glass bulb mercury thermometers were the standard device to measure body temperature. Today, mercury thermometers are prohibited in many countries due to the hazardous effects of mercury and the risk of exposure if the glass bulb breaks. In general,...
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Temperature Measurement Sites01:14

Temperature Measurement Sites

4.3K
A thermometer measures body temperature. The common sites for measuring body temperature are the oral cavity, axillary region, temporal artery, and skin surface, such as the forehead, abdomen, and axilla. True core body temperature is assessed in the rectum, tympanic membrane, pulmonary artery, esophagus, and urinary bladder.
Oral: When assessing oral temperature, the thermometer tip should be placed under the tongue in the posterior sublingual pocket. It offers accurate readings and can be...
4.3K

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使用温度控制的频率混合磁探测基于便携式现场测试平台的异热放大.

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  • 1Institute of Biological Information Processing: Bioelectronics (IBI-3), Forschungszentrum Jülich, 52428 Jülich, Germany.

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概括

本研究展示了如何使用频率混合磁检测 (FMMD) 控制磁核酸检测的温度. 这种方法可以在移动,资源有限的环境中实现可靠的DNA放大和检测.

关键词:
频率混合磁检测检测频率混合磁检测磁性纳米粒子是一种磁性纳米粒子.关心点检测检测的测试点.复合酶聚合酶放大复合酶.热一次性参数模型的模型.

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科学领域:

  • 生物技术是生物技术.
  • 生物感应是一种生物感应.
  • 分子诊断学 分子诊断

背景情况:

  • 使用频率混合磁检测 (FMMD) 进行敏感磁核酸 (NA) 检测,需要对放大 NA 样本进行精确的温度控制.
  • 重组酶聚合酶放大 (RPA) 是NA放大的一个关键步骤,但需要稳定的温度条件.

研究的目的:

  • 调查将RPA与移动FMMD设置集成为温度控制NA放大器的可行性.
  • 在不同的环境条件下,开发和验证FMD传感器单元内的精确温度控制的热模型.

主要方法:

  • 利用FMMD低频 (LF) 激发信号产生的固有热量,通过脉冲宽度调制 (PWM) 控制.
  • 开发了稳态和动态响应模型,以预测样本位置的热行为.
  • 在FMMD传感器单元内验证的RPA性能与标准温度控制的水浴相比.

主要成果:

  • 在FMMD传感器单元内成功控制了RPA的温度,实现了与水浴相比的性能.
  • 开发了精确的热模型:对狭窄的温度范围进行线性推断,对更广泛的条件进行一次性参数模型 (LPM).
  • 证实了PWM在使用LF激发信号的热量来控制温度的有效性.

结论:

  • 集成的FMMD系统与基于PWM的温度控制使核酸放大可靠的RPA成为可能.
  • 开发的热模型准确地预测样本温度,方便在多种 Point-of-Care (PoC) 环境中操作.
  • 这种方法对使用FMMD在资源有限的环境中进行核酸放大和磁探测具有前景.