开发一种稳定解决方案,以在室温下长期储存菌体,并应用于控制食源性病原体
Eo-Jin Kim1,2, Min-Cheol Lim1, Min-Ah Woo1
1Food Safety and Distribution Research Group, Korea Food Research Institute, Wanju-gun 55365, Republic of Korea.
Viruses
|July 27, 2024
概括
研究人员开发了一种新型的稳定溶液,以增强菌体 (菌体) 稳定性,用于食品安全应用. 这种溶液在室温下维持了48周的菌体活动,有效地减少了蔬菜上的病原体.
科学领域:
- 食品微生物学 食品微生物学
- 菌体技术的细菌体技术.
- 抗微生物药物是一种抗菌剂.
背景情况:
- 菌体 (菌体) 是感染细菌的病毒,并显示为食品安全的天然抗菌剂的希望.
- 由于稳定性和监管问题,目前的菌体存储方法限制了它们在食品中的直接应用.
- 开发稳定,适用于食品的菌体配方对于它们在食品行业的商业化至关重要.
研究的目的:
- 开发一种稳定菌体 (菌体) 的稳定溶液,以维持它们在室温下的活动,以便直接应用于食品中.
- 评估菌体处理在减少受污染蔬菜上的病原细菌的有效性.
- 评估基于菌体的食品安全干预措施的商业化潜力.
主要方法:
- 菌体被储存在蒸水和含有糖和盐的新型稳定溶液中.
- 菌体的稳定性在室温下在48周内被评估.
- 用两种应用方法测试了菌体在污染蔬菜上减少大肠杆菌O157:H7的有效性:直接洗和菌体涂层容器系统.
主要成果:
- 与蒸水中的菌体相比,存储在稳定溶液中的菌体表现出显著改善的稳定性,在室温下持续长达48周,与蒸水中的菌体相比.
- 用含有菌体的自来水清洗受污染的蔬菜可以减少*大肠杆菌*O157:H7的数量超过90%.
- 一个菌体涂层的容器系统也实现了显著的90%的减少 *E. coli* O157:H7 在蔬菜上的污染.
结论:
- 开发的稳定溶液有效地保持了室温的菌体活动,使其能够直接应用于食品中.
- 基于菌体的干预措施,包括直接洗和涂层容器,在减少蔬菜上的致病性大肠杆菌方面非常有效.
- 这项研究提出了一种具有成本效益和商业可行性的方法,用于使用菌体提高食品安全.
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