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基于RNA的疫苗调节的局部和全身免疫反应

Fatemeh Fazel¹, Ayumi Matsuyama-Kato¹, Mohammadali Alizadeh¹

¹Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, ON N1G 2W1, Canada.

|July 27, 2024

概括

这项研究表明,针对马雷克病 (MD) 病毒的mRNA疫苗可以激活的免疫反应. 两剂疫苗诱导抗病毒状态,增强对MDV的保护.

科学领域:

兽医免疫学兽医免疫学
分子病毒学分子病毒学
疫苗开发疫苗开发

背景情况:

马雷克病 (MD) 是一种严重的淋巴增殖性疾病,由马雷克病病毒 (MDV) 引起.
目前的MD疫苗限制疾病,但不能防止MDV的复制或传播.
需要新的疫苗策略来增强对MDV的宿主抗病毒反应.

研究的目的:

在肌肉内注射编码MDV gB和pp38抗原的mRNA疫苗后评估宿主免疫反应.
评估主要和主要增强疫苗接种策略对先天性和适应性免疫基因表达的影响.
为了确定在接种疫苗后的中诱导抗病毒状态.

主要方法:

被肌肉内注射一种编码MDV gB和pp38蛋白质的mRNA疫苗,低剂量和高剂量.
使用了主要和主要增强疫苗接种策略.
对脏和肺部的I型和II型干扰素 (IFN),干扰素刺激基因 (ISG) 和关键抗病毒细胞因子 (IL-1β,IL-2) 的转录分析是在36小时内和二次免疫接种后进行的.

主要成果:

在初级免疫接种后36小时内,在脏和肺部观察到MDA5,Myd88,IFN-α,IFN-β,IFN-γ,IRF7,OAS,Mx1和IL-2的显著上调.
二次免疫接种进一步增加了这些基因在肺部的表达.
在脏中,二次免疫导致IFN-γ,MDA5,MyD88,Mx1和OAS的显著上调.

结论:

编码gB和pp38抗原的MDV mRNA疫苗有效地激活了的先天和适应性免疫反应.
一个主要提升策略增强了抗病毒基因表达的诱导,特别是在肺部.
这项研究表明,这种mRNA疫苗有潜力诱导针对马雷克病病毒的抗病毒状态.

关键词:

马雷克的疾病病毒病毒.这些都是肉肉.细胞因子细胞因子免疫反应的免疫反应.这是mRNA疫苗.

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