稳定USP7的HIPK2促进高葡萄糖诱导的内皮细胞功能障碍,加速糖尿病足
Huimin Huang1, Yangyong Huang2
1Burn & Plastic & Wound Surgery Department, The First College of Clinical Medical Science, China Three Gorges University, Yichang, China.
Archives of physiology and biochemistry
|July 27, 2024
概括
USP7稳定HIPK2,促进高葡萄糖诱导的内皮细胞功能障碍,加速糖尿病足 (DFU). 针对这个USP7/HIPK2轴可能为DFU提供新的治疗策略.
科学领域:
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
- 生物化学 生物化学
背景情况:
- 糖尿病足 (DFU) 是糖尿病的一个重要并发症.
- 了解DFU病原体的分子机制对于开发有效的治疗方法至关重要.
研究的目的:
- 调查主体主体相互作用蛋白激酶2 (HIPK2) 在糖尿病足 (DFU) 的分子机制中的作用.
- 阐明USP7和HIPK2在高葡萄糖诱导的内皮细胞功能障碍中的调节关系.
主要方法:
- 使用高葡萄糖 (HG) 诱导的人类静脉内皮细胞 (HUVECs) 建立了一个DFU细胞模型.
- 评估细胞功能,包括增殖,入侵,迁移,血管新生和细胞亡,使用各种测定方法 (CCK8,EDU,流细胞计,transwell,伤口愈合,管形成).
- 量化基因和蛋白质表达,使用定量实时PCR和西方Blot.
主要成果:
- 高葡萄糖 (HG) 损害了HUVEC功能,增加了细胞亡,抑制了增殖,入侵,迁移和血管生成.
- 在DFU中,HIPK2被过度表达,其敲击改善了HG诱导的HUVEC功能障碍.
- USP7通过降低其无处不在性来稳定HIPK2,USP7/HIPK2轴抑制PI3K/AKT通路.
结论:
- 稳定USP7的HIPK2会加剧高葡萄糖诱导的内皮细胞功能障碍,导致糖尿病足 (DFU) 的进展.
- USP7/HIPK2相互作用代表了DFU治疗的潜在治疗标.
相关概念视频
01:58Cell Specific Gene Expression
13.5K
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
13.5K
01:24Regulation of Angiogenesis and Blood Supply
2.5K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
2.5K
01:27Glucose Homeostasis: Pancreatic Islets and Insulin Secretion
1.2K
The pancreatic islets comprising only 1%-2% of the volume are highly vascularized and innervated mini-organs. They contain five endocrine cell types, including β cells that secrete insulin, which is synthesized as a single polypeptide chain, preproinsulin, processed to proinsulin, and finally to insulin and C-peptide. This process is complex and regulated, involving the Golgi complex, the endoplasmic reticulum, and the secretory granules of the β cell.
Insulin and C-peptide are...
Insulin and C-peptide are...
1.2K