相关实验视频

Updated: Jun 18, 2025

Application of CRISPR Interference CRISPRi for Gene Silencing in Pathogenic Species of Leptospira

Application of CRISPR Interference CRISPRi for Gene Silencing in Pathogenic Species of Leptospira

Published on: August 14, 2021

在Klebsiella variicola中基于CRISPR-Cas的原生可编程多重基因抑制

Zhifeng Mo¹, Siying Lin¹, Ting Li¹

¹State Key Laboratory of Green Pesticide, Guangdong Province Key Laboratory of Microbial Signals and Disease Control, Integrative Microbiology Research Centre, South China Agricultural University, Guangzhou, China.

Biotechnology letters

|July 27, 2024

概括

研究人员开发了一种新的CRISPR-Cas工具,用于克莱布西埃拉 (Klebsiella variicola) 的基因抑制. 该系统可实现向基因沉默和多重编辑,有助于理解病变发生和代谢工程.

科学领域:

微生物学微生物学
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

克莱布西拉 (Klebsiella variicola) 是一种具有生物技术潜力的机会性病原体.
由于缺乏遗传工具,对其病原和有益活动的理解受到阻碍.
在K. variicola菌株KV-1中发现了一种原生类型I-E CRISPR-Cas系统.

研究的目的:

描述K. variicola KV-1中的原生类型I-E CRISPR-Cas系统的特征.
为了重新利用系统进行向基因抑制.
开发一种可编程多重基因抑制工具,用于K. variicola.

主要方法:

在K. variicola KV-1中识别和表征I-E型CRISPR-Cas系统.
设计和引入一个具有迷你CRISPR阵列的准等离子体.
具体基因表达抑制和多重基因抑制的证明.

主要成果:

在KV-1的原生类型I-E CRISPR-Cas系统中,保留了crRNA引导的状DNA结合活性.
通过向等离子体重新利用系统,实现了高效和特定的向基因抑制.
通过使用编码多个crRNAs的单个向等离子体,成功证明了多重基因抑制.

结论:

开发了一种基于CRISPR-Cas的新型工具,用于K. variicola中的可编程多重基因抑制.
这种工具有助于研究K. variicola的病原性.
该工具使代谢工程能够生产有价值的生物产品.

关键词:

克莱布西拉 (Klebsiella variicola) 是一种植物.这就是CRISPR-Cas.基因抑制基因抑制迷你CRISPR是什么意思

更多相关视频

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells

Published on: June 16, 2017

A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization

A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization

Published on: September 2, 2021

相关概念视频

CRISPR and crRNAs

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...

CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...

Conservative Site-specific Recombination and Phase Variation

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...