相关实验视频

Updated: Jun 18, 2025

Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability

Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability

Published on: April 22, 2016

通过Arthrobacter sp.有效地利用cAMP救援合成的增强型山丁利用. CCTCC 2013431 通过克桑氧化酶抑制的方法

Baofeng Chen¹, Hai Tan¹, Chang Li²

¹School of Life Sciences, Henan Institute of Science and Technology, 90 Hualan Road, Xinxiang, 453003, Henan, China.

Biotechnology letters

|July 27, 2024

概括

添加素增强循环腺单酸盐 (cAMP) 挽救合成通过抑制桑丁氧化酶,减少 hypoxanthine 分解和细胞损伤. 这提高了cAMP的生产效率和发酵期间的细胞活力.

科学领域:

生物技术是生物技术.
代谢工程是代谢工程.
生物化学工程生物化学工程

背景情况:

循环腺单酸盐 (cAMP) 合成依赖于救援通路,这些通路可以通过低素代谢来抑制.
丁氧化酶在使用低丁时的活性导致低转化率和细胞活力降低.
提高cAMP救援合成需要策略来减轻低氨酸代谢的负面影响.

研究的目的:

为了研究氨酸在发酵过程中对cAMP救援合成的影响.
评估黄素在改善低氨酸转化和细胞活性的潜力.
阐明黄素对细胞代谢和酶活性作用的潜在机制.

主要方法:

在7L生物反应器中使用素作为激活剂的cAMP救援通路的发酵.
用单独添加酸盐,黄素或低素进行发酵性能比较分析.
对多种生理指标的测定,包括酶活性,代谢物水平,基因表达和活性氧物种 (ROS) 生成.

主要成果:

添加素显著提高了72小时的cAMP发酵性能,超过了对照和添加酸盐的批量.
素的素转换比率和cAMP含量较高,尽管单独使用素的初始生产力增加是短暂的.
素抑制了山丁氧化酶,降低了素的分解和ROS生成,导致ATP/AMP,NADH/NAD+和NADP/NADP+比率的增加.

结论:

素有效地抑制了山丁氧化酶活性,从而降低了cAMP救援合成期间的低山丁分解和ROS生成.
添加丁醇可以提高素的转化效率,并减少细胞损伤,从而提高和持续的cAMP生产.
氨酸代表了优化生物技术应用中cAMP救援合成的有希望的策略.

关键词:

低素的转换转换方法卢特奥林是什么? 卢特奥林是什么?氧化应激是一种氧化应激.亚丁氧化酶是一种丁氧化酶.

更多相关视频

Methods to Inhibit Bacterial Pyomelanin Production and Determine the Corresponding Increase in Sensitivity to Oxidative Stress

Methods to Inhibit Bacterial Pyomelanin Production and Determine the Corresponding Increase in Sensitivity to Oxidative Stress

Published on: August 31, 2015

Phage-Mediated Genetic Manipulation of the Lyme Disease Spirochete Borrelia burgdorferi

Phage-Mediated Genetic Manipulation of the Lyme Disease Spirochete Borrelia burgdorferi

Published on: September 28, 2022

相关概念视频

The Electron Transport Chain

The Electron Transport Chain

The electron transport chain or oxidative phosphorylation is an exothermic process in which free energy released during electron transfer reactions is coupled to ATP synthesis. This process is a significant source of energy in aerobic cells, and therefore inhibitors of the electron transport chain can be detrimental to the cell's metabolic processes.
Inhibitors of the electron transport chain
Rotenone, a widely used pesticide, prevents electron transfer from Fe-S cluster to ubiquinone or Q...

Allosteric Proteins-ATCase

Allosteric Proteins-ATCase

Binding sites linkages can regulate a protein's function. For example, enzyme activity is often regulated through a feedback mechanism where the end product of the biochemical process serves as an inhibitor.
Aspartate transcarbamoylase (ATCase) is a cytosolic enzyme that catalyzes the condensation of L-aspartate and carbamoyl phosphate to N-carbamoyl-L-aspartate. This reaction is the first step in pyrimidine biosynthesis. UTP and CTP, the end products of the pyrimidine synthesis...